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Nicotine and Cotinine in Urine (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the levels of nicotine and its metabolite, cotinine, in your urine to assess recent exposure to tobacco smoke.

Quick summary

["Helps track smoking habits and potential exposure to secondhand smoke."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
28555-1
Short code
Nicotine+Cotinine Ur Ql Cfm
Synonyms
Nicotine+Cotinine Ur Ql Cfm
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
confirm
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Matters

Nicotine is the primary psychoactive compound in tobacco, while cotinine is a breakdown product that remains in the body longer. Both are detected in urine shortly after exposure, making this test useful for monitoring recent smoking or environmental tobacco exposure.

How the Test Works

Your urine sample is analyzed using a sensitive laboratory method to measure the concentrations of nicotine and cotinine. This helps healthcare providers determine the extent of exposure over a short period.

What to Expect Before the Test

No special preparation is needed. Collect a urine sample as directed by your healthcare provider, typically a random sample or a timed 24-hour collection (if specified). Avoid smoking or using tobacco products before testing if instructed.

Possible Results and Interpretation

Results are interpreted based on the measured concentrations of nicotine and cotinine. Higher levels may indicate recent smoking or exposure, while lower levels suggest minimal or no exposure. Your healthcare provider will discuss findings with you.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Urine Nicotine & Cotinine

Frequently asked questions

How might this test help my healthcare provider?
This test can help confirm recent tobacco use or exposure, especially in individuals who may not report smoking habits accurately. It may also be used in research, workplace safety assessments, or monitoring for secondhand smoke exposure in sensitive populations like children or nonsmokers.
What does it mean if my results are positive?
Positive results indicate detectable levels of nicotine or cotinine, suggesting recent exposure to tobacco products. This could mean you smoked, were exposed to secondhand smoke, or used other tobacco-containing products. Your provider will discuss next steps, such as counseling or support for quitting.
Can I still smoke or use tobacco before this test?
If your provider instructed a specific test timing, avoid smoking or using tobacco products for at least 24 hours before collection to ensure accurate results. Follow their guidance to interpret findings correctly.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy