Plasma Free Testosterone by Immunoassay (Screening)
This test measures the amount of free (unbound) testosterone in your blood, which is the active form of testosterone that circulates freely in your plasma.
["Free testosterone helps assess hormone balance, especially in conditions affecting sex hormone levels."]
Key facts at a glance
- LOINC
- 73967-2
- Short code
- NIP fet aneu Pnl Plas.cfDNA
- Synonyms
- NIP fet aneu Pnl Plas.cfDNA
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 136
- Ref high
- 145
- Critical range
- 120 N/A – 160 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- CPT
- 84295
- SNOMED
- 110741000
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why This Test Matters
Free testosterone is important because it reflects the biologically active portion of testosterone that your body uses. Unlike total testosterone, free testosterone isn’t bound to proteins and is directly available to your cells. This can be particularly useful for diagnosing conditions like hypogonadism (low testosterone) or evaluating symptoms related to hormonal imbalances, such as irregular menstrual cycles or low libido.
How the Test Works
Your blood sample is analyzed using an immunoassay method, which detects the presence of free testosterone molecules in your plasma. This method is highly sensitive and specific, helping to accurately measure the concentration of free testosterone without interference from other substances.
Sample Collection and Preparation
A blood sample is drawn from a vein, typically in your arm. The blood is processed to separate the plasma (the liquid part containing hormones and other substances) from the blood cells. This plasma is then sent to the lab for testing.
What to Expect Before the Test
No special preparation is needed for this test. You may have some mild discomfort or bruising at the injection site, but this usually fades quickly. If you’re on certain medications that affect hormones, let your healthcare provider know before the test.
Reference range
Adult: 136-145 mmol/LN/A
No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings1
Short name
Free testosterone screening
Frequently asked questions
What Does a Low Free Testosterone Level Mean?
What Does a High Free Testosterone Level Mean?
How Often Should This Test Be Done?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy