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Plasma Free Testosterone by Immunoassay (Screening)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of free (unbound) testosterone in your blood, which is the active form of testosterone that circulates freely in your plasma.

Quick summary

["Free testosterone helps assess hormone balance, especially in conditions affecting sex hormone levels."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
73967-2
Short code
NIP fet aneu Pnl Plas.cfDNA
Synonyms
NIP fet aneu Pnl Plas.cfDNA
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
136
Ref high
145
Critical range
120 N/A – 160 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84295
SNOMED
110741000
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Matters

Free testosterone is important because it reflects the biologically active portion of testosterone that your body uses. Unlike total testosterone, free testosterone isn’t bound to proteins and is directly available to your cells. This can be particularly useful for diagnosing conditions like hypogonadism (low testosterone) or evaluating symptoms related to hormonal imbalances, such as irregular menstrual cycles or low libido.

How the Test Works

Your blood sample is analyzed using an immunoassay method, which detects the presence of free testosterone molecules in your plasma. This method is highly sensitive and specific, helping to accurately measure the concentration of free testosterone without interference from other substances.

Sample Collection and Preparation

A blood sample is drawn from a vein, typically in your arm. The blood is processed to separate the plasma (the liquid part containing hormones and other substances) from the blood cells. This plasma is then sent to the lab for testing.

What to Expect Before the Test

No special preparation is needed for this test. You may have some mild discomfort or bruising at the injection site, but this usually fades quickly. If you’re on certain medications that affect hormones, let your healthcare provider know before the test.

Reference range

Adult: 136-145 mmol/LN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Free testosterone screening

Frequently asked questions

What Does a Low Free Testosterone Level Mean?
A low free testosterone level may indicate conditions like pituitary disorders, primary hypogonadism, or chronic illnesses that affect hormone production. Your doctor may recommend further testing or treatment to address the underlying cause.
What Does a High Free Testosterone Level Mean?
High free testosterone levels can be linked to conditions like PCOS, androgen-producing tumors, or certain medications. It may also cause symptoms like acne, excessive hair growth, or menstrual irregularities in women, or erectile dysfunction in men.
How Often Should This Test Be Done?
The frequency of this test depends on your medical history, symptoms, and ongoing treatment. Your doctor will determine how often to repeat the test based on your individual needs, such as monitoring hormone therapy or managing a diagnosed condition.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy