Serum/plasma concentration of free testosterone (immunoassay)
This test measures the amount of free (unbound) testosterone in your blood, which is the active form of the hormone that helps regulate male and female sex characteristics.
["Assesses your body’s free testosterone levels for hormonal balance and potential conditions like infertility or hormone-related disorders."]
Key facts at a glance
- LOINC
- 96977-4
- Short code
- NIP fet aneu Pnl WBC.DNA+cfDNA
- Synonyms
- NIP fet aneu Pnl WBC.DNA+cfDNA
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 136
- Ref high
- 145
- Critical range
- 2 N/A – 30 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- dosage-of-chromosome-specific-cf-dna
- CPT
- 84295
- SNOMED
- 110741000
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why This Test Matters
Free testosterone is the biologically active form of testosterone that circulates freely in your blood. Unlike total testosterone, which includes both free and bound forms, free testosterone is directly available to your cells. This test helps doctors evaluate your hormone health, especially in conditions affecting sex hormones, fertility, or symptoms like low libido or irregular menstrual cycles.
What to Expect Before the Test
No special preparation is needed for this test. You may have a blood draw at your doctor’s office or a lab. If you’re taking medications that affect hormones, like certain birth control pills or steroids, let your healthcare provider know before the test.
Common Reasons for Testing
Your doctor might order this test to check for hormonal imbalances, diagnose conditions like hypogonadism (low testosterone) or hyperandrogenism (high testosterone), or monitor treatment for hormone-related disorders. It’s also used in fertility evaluations or to assess symptoms like fatigue, mood changes, or changes in body hair.
What the Results Mean
Results are compared to reference ranges for your age, sex, and health status. While specific ranges vary by lab, low free testosterone may indicate conditions like pituitary disorders or androgen resistance, while very high levels could suggest tumors or other hormonal issues. Your doctor will interpret results in the context of your overall health.
Reference range
Adult: 136-145 mmol/LN/A
No special preparation required. Stay well hydrated.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance
Short name
Free testosterone level
Frequently asked questions
How does free testosterone differ from total testosterone?
What symptoms might indicate abnormal free testosterone levels?
Can diet or exercise affect free testosterone levels?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy