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Serum/plasma concentration of free testosterone (immunoassay)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of free (unbound) testosterone in your blood, which is the active form of the hormone that helps regulate male and female sex characteristics.

Quick summary

["Assesses your body’s free testosterone levels for hormonal balance and potential conditions like infertility or hormone-related disorders."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
96977-4
Short code
NIP fet aneu Pnl WBC.DNA+cfDNA
Synonyms
NIP fet aneu Pnl WBC.DNA+cfDNA
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
136
Ref high
145
Critical range
2 N/A – 30 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
dosage-of-chromosome-specific-cf-dna
CPT
84295
SNOMED
110741000
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Matters

Free testosterone is the biologically active form of testosterone that circulates freely in your blood. Unlike total testosterone, which includes both free and bound forms, free testosterone is directly available to your cells. This test helps doctors evaluate your hormone health, especially in conditions affecting sex hormones, fertility, or symptoms like low libido or irregular menstrual cycles.

What to Expect Before the Test

No special preparation is needed for this test. You may have a blood draw at your doctor’s office or a lab. If you’re taking medications that affect hormones, like certain birth control pills or steroids, let your healthcare provider know before the test.

Common Reasons for Testing

Your doctor might order this test to check for hormonal imbalances, diagnose conditions like hypogonadism (low testosterone) or hyperandrogenism (high testosterone), or monitor treatment for hormone-related disorders. It’s also used in fertility evaluations or to assess symptoms like fatigue, mood changes, or changes in body hair.

What the Results Mean

Results are compared to reference ranges for your age, sex, and health status. While specific ranges vary by lab, low free testosterone may indicate conditions like pituitary disorders or androgen resistance, while very high levels could suggest tumors or other hormonal issues. Your doctor will interpret results in the context of your overall health.

Reference range

Adult: 136-145 mmol/LN/A

Specimen: serum

No special preparation required. Stay well hydrated.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Free testosterone level

Frequently asked questions

How does free testosterone differ from total testosterone?
Free testosterone is the portion of testosterone that isn’t bound to proteins in your blood and is therefore biologically active. Total testosterone includes both free and bound forms, so it’s a broader measure. Free testosterone is often preferred for assessing hormone function because it reflects the actual amount available to your body’s tissues.
What symptoms might indicate abnormal free testosterone levels?
Symptoms of low free testosterone may include low libido, erectile dysfunction, fatigue, mood changes, reduced muscle mass, or thinning hair. Symptoms of high free testosterone in women may include irregular periods, acne, or excessive body hair growth. In men, high levels could cause breast enlargement or mood swings. Always consult your doctor if you experience these symptoms.
Can diet or exercise affect free testosterone levels?
Yes, lifestyle factors like diet, exercise, and weight management can influence testosterone levels. Eating a balanced diet rich in zinc, vitamin D, and healthy fats, along with regular physical activity, may support healthy hormone levels. Avoiding excessive alcohol and smoking can also help maintain stable hormone balance.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy