Total Protein with Albumin and Globulin Separation (Serum)
This test measures total protein in your blood and breaks it down into albumin and globulin fractions, which help assess protein balance and potential liver or kidney issues.
["Your blood protein levels can signal health problems like liver disease, kidney problems, or nutritional deficiencies."]
Key facts at a glance
- LOINC
- 41846-7
- Short code
- N men sg A+B+C+W135+Y+EcK1 Ag Ur Ql Aggl
- Synonyms
- N men sg A+B+C+W135+Y+EcK1 Ag Ur Ql Aggl
- Category
- chemistry
- Sample
- urine
- Container
- Sterile urine container
- Units
- N/A
- Ref low
- 22
- Ref high
- 198
- Critical range
- — – 10000 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- aggl
- CPT
- 82553
- Specimen volume
- 10 mL
- Stability
- Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 4–24 hours
- Order
- routine
Why This Test Matters
Protein is essential for many bodily functions, including transporting nutrients, maintaining fluid balance, and supporting immune responses. Albumin, the most abundant protein in blood, helps keep fluids in circulation, while globulins assist with immune function and blood clotting. Abnormal levels may indicate conditions like liver disease, kidney dysfunction, or malnutrition.
How the Test Works
Blood is drawn and separated into components. The total protein is measured directly, while albumin and globulin levels are often derived from the total protein result and additional calculations or electrophoresis if detailed separation is needed.
What to Expect Before the Test
No special preparation is usually required, but you may be asked to fast for 8–12 hours if albumin is being specifically measured for accuracy. Wear a comfortable shirt for easy access to your arm.
Possible Results and Their Meaning
Total protein and albumin levels are often reported together. Low levels may suggest malnutrition, liver disease, or kidney problems, while high levels could indicate dehydration, inflammation, or conditions like multiple myeloma. Globulin levels alone may not be reported unless further testing is done.
Reference range
Adult males: 22-198 U/L; Females: 22-198 U/LN/A
No special preparation required. Inform provider of any chest pain or symptoms.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Abnormal protein levels can signal underlying health issues. For example, low albumin may indicate liver disease or poor absorption of nutrients, while elevated globulins could point to chronic infections, autoimmune disorders, or certain cancers. Your doctor will use these results to guide further evaluation or treatment.
Short name
Protein profile
Frequently asked questions
What does a low total protein level mean?
How long does it take to get my protein test results?
Can I eat or drink before this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy