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Cerebrospinal Fluid (CSF) Protein Electrophoresis

Published: August 9, 2026Updated: August 16, 2026

This test measures the different types of proteins found in your cerebrospinal fluid (the fluid surrounding your brain and spinal cord). It helps identify abnormalities in protein distribution that may signal conditions like multiple sclerosis, infections, or tumors.

Quick summary

["This test helps diagnose and monitor neurological conditions by analyzing protein patterns in your spinal fluid."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
31914-5
Short code
N men sg B+E coli K1 Ag CSF Ql
Synonyms
N men sg B+E coli K1 Ag CSF Ql
Category
chemistry
Sample
csf
Container
Sterile tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
1-2 mL
Stability
Immediate analysis required, Refrigerated: 1h
Hemolysis max
200
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if you have symptoms like headaches, vision changes, numbness, weakness, or trouble with coordination. It’s also used to check for conditions like multiple sclerosis, meningitis, or brain tumors, especially when other tests don’t provide clear answers.

How the Test Works

A small amount of cerebrospinal fluid is collected through a procedure called a lumbar puncture (spinal tap). The fluid is then analyzed using electrophoresis, a method that separates proteins based on their size and electrical charge to identify patterns.

Sample Collection and Preparation

No special preparation is needed before the test. You’ll lie on your side and have a needle inserted into your lower back to collect the fluid. The procedure is quick but may cause mild discomfort.

What to Expect After the Test

After the procedure, you’ll rest for a few minutes before standing up slowly. You may experience mild back pain or headache, which can be managed with over-the-counter pain relievers. Avoid strenuous activity for 24 hours.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

CSF Protein Profile

Frequently asked questions

How long does it take to get results from this test?
Results are typically available within 1 to 3 business days, depending on your lab’s processing time.
Can I eat or drink before this test?
No special diet is required, but you may drink water as usual. Avoid caffeine or alcohol before the procedure to reduce the risk of headaches afterward.
Is the lumbar puncture procedure painful?
The procedure itself is uncomfortable but not usually painful. You may feel pressure or a slight sting during the needle insertion. Most people describe it as similar to a mild backache afterward.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy