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N-Methylnicotinamide (NMN) in Urine

Published: August 9, 2026Updated: August 16, 2026

This test measures the presence of N-methylnicotinamide (NMN), a metabolite of niacin (vitamin B3), in your urine. It may be used to assess how well your body processes niacin.

Quick summary

["This test helps evaluate niacin metabolism and may be part of broader studies on vitamin B3 metabolism."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
74535-6
Short code
N men sg X DNA Spec Ql NAA+probe
Synonyms
N men sg X DNA Spec Ql NAA+probe
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Does This Test Measure?

N-methylnicotinamide (NMN) is a breakdown product of niacin (vitamin B3), a water-soluble vitamin essential for energy production and nerve function. Your body converts niacin into NMN as part of its normal metabolic process. This test detects the amount of NMN excreted in your urine over a 24-hour period.

Why Might Your Doctor Order This Test?

Doctors may request this test if they suspect abnormal niacin metabolism, particularly in conditions involving niacin deficiency, genetic disorders affecting vitamin B3 processing, or as part of research studies on metabolic pathways.

Sample Collection and Preparation

For this test, a 24-hour urine collection is typically required. You’ll collect all urine produced during the specified period in a special container. The sample is then sent to the lab for analysis.

What to Expect Before the Test

No special preparation is usually needed unless instructed by your healthcare provider. Ensure you’re drinking enough fluids to maintain normal urine output, but avoid excessive intake that might dilute the sample.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Abnormal NMN levels may indicate underlying metabolic issues, such as niacin deficiency or genetic disorders affecting vitamin B3 metabolism. Elevated levels could also be part of broader research into how the body processes vitamins and nutrients. Your doctor will interpret results in the context of your overall health and medical history.

Short name

NMN Urine Test

Frequently asked questions

What Are Common Causes of Abnormal Results?
Abnormal NMN levels can result from conditions affecting niacin metabolism, such as rare genetic disorders or certain medications that influence vitamin B3 processing. However, results should always be reviewed by a healthcare provider.
How Is This Test Different from Other Vitamin B3 Tests?
Unlike blood tests for niacin or its metabolites (e.g., nicotinamide), this test specifically measures NMN in urine. It provides insight into how efficiently your body converts niacin into NMN for excretion.
Does This Test Require Special Dietary Restrictions?
No strict dietary restrictions are typically required, but you may be advised to avoid high-niacin foods or supplements unless directed by your doctor to prevent interference with test accuracy.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy