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Cerebrospinal Fluid Protein by Manual Qualitative Analysis (Bromocresol Green)

Published: August 9, 2026Updated: August 16, 2026

This test checks for protein levels in your cerebrospinal fluid (CSF), the fluid surrounding your brain and spinal cord. It may be used to detect conditions affecting the central nervous system.

Quick summary

["Protein in CSF can signal inflammation, infection, or structural issues in the brain or spinal cord."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
77356-4
Short code
Nonhematop stem cell NFr CSF Manual
Synonyms
Nonhematop stem cell NFr CSF Manual
Category
chemistry
Sample
csf
Container
Sterile tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
manual-count
Specimen volume
1-2 mL
Stability
Immediate analysis required, Refrigerated: 1h
Hemolysis max
200
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your doctor may recommend this test if you have symptoms like headaches, neck stiffness, vision changes, or neurological issues. It could also be part of a broader evaluation for conditions like meningitis, multiple sclerosis, or tumors.

How the Test Works

A small sample of your CSF is collected via a spinal tap (lumbar puncture). The sample is then manually tested using bromocresol green dye to visually assess protein concentration. This method helps identify elevated protein levels without needing precise measurements.

What to Expect Before the Test

No special preparation is needed, but you may be asked to avoid certain medications or activities that could affect results. Discuss any concerns with your healthcare provider.

What to Expect During the Test

The procedure involves inserting a needle into your lower back to collect CSF. You may feel pressure or mild discomfort, but it usually doesn’t cause significant pain. You’ll lie on your side for a short time afterward.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

CSF Protein Screen (Manual BCG)

Frequently asked questions

What does a positive or abnormal result mean?
An abnormal result may mean your CSF contains higher-than-normal protein, which could signal an underlying issue like infection, inflammation, or a neurological disorder. Your doctor will interpret results in the context of your symptoms and other tests.
How long will it take to get results?
Results from this manual test are typically available within hours, depending on lab processing times. Follow-up with your doctor to discuss findings.
Can I eat or drink before this test?
No special dietary restrictions apply, but avoid caffeine or alcohol before the procedure to minimize discomfort during the spinal tap.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy