Skip to content

Cerebrospinal Fluid (CSF) Protein Electrophoresis (Manual Zone Separation)

Published: August 9, 2026Updated: August 17, 2026

This test measures the different protein fractions in your cerebrospinal fluid (CSF) to help identify abnormalities in protein distribution, which can indicate conditions like multiple sclerosis, infections, or other neurological disorders.

Quick summary

["This test helps diagnose and monitor conditions affecting your brain and spinal cord by analyzing protein patterns in CSF."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
40631-4
Short code
nRBC CSF Manual-Rto
Synonyms
nRBC CSF Manual-Rto
Category
chemistry
Sample
csf
Container
Sterile tube
Units
N/A
Ref low
4.2
Ref high
5.9
Critical range
2 N/A – 7 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
manual-count
CPT
85041
SNOMED
900000000000003001
Specimen volume
1-2 mL
Stability
Immediate analysis required, Refrigerated: 1h
Hemolysis max
200
TAT
4–24 hours
Order
routine

What Does This Test Measure?

The test separates CSF proteins into distinct zones (e.g., albumin, globulins, and other protein fractions) using manual zone electrophoresis. This helps identify abnormal protein patterns that may suggest underlying health issues.

Why Might You Need This Test?

Your healthcare provider may order this test if you have symptoms like headaches, vision changes, numbness, or neurological issues that could be linked to protein abnormalities in the CSF.

How Is the Sample Collected?

A small amount of CSF is collected through a procedure called lumbar puncture (spinal tap). The sample is then analyzed in a lab to separate and measure protein fractions.

What Are Common Uses?

This test is often used to diagnose conditions such as multiple sclerosis, meningitis, Guillain-Barré syndrome, or other inflammatory or infectious diseases affecting the central nervous system.

Reference range

Adult males: 4.2-5.9; Females: 3.8-5.1 x 10^12/LN/A

Specimen: csf

No special preparation required. Stay well hydrated.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical significance: Abnormal protein patterns in CSF can indicate inflammation, infections, or demyelination in the brain or spinal cord. For example, elevated immunoglobulin bands may suggest autoimmune diseases, while high albumin levels could point to a blood-brain barrier issue.

Short name

CSF Protein Electrophoresis

Frequently asked questions

What does a normal result mean?
A normal result typically shows balanced protein distribution across zones, with no unusual spikes or patterns. However, individual results depend on the specific clinical context and your healthcare provider’s assessment.
How long does it take to get results?
Results are usually available within 1–3 business days, depending on lab processing times and your healthcare provider’s workflow.
Can I eat or drink anything before this test?
No special diet or preparation is needed before this test. However, inform your provider about any medications or supplements you’re taking, as they may affect results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy