Skip to content

Nitric Oxide in Serum (as Nitrite/Nitrate)

Published: August 9, 2026Updated: August 17, 2026

This test measures the presence of nitric oxide, a chemical produced by your body that helps regulate blood flow and inflammation. It may be checked to assess vascular health or response to certain conditions.

Quick summary

["Nitric oxide helps control blood pressure and blood vessel function; low levels may signal potential vascular issues."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
110215-1
Short code
NT-proBNP Msmt SerPl
Synonyms
NT-proBNP Msmt SerPl
Category
coagulation
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0
Ref high
100
Critical range
— – 500 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83880
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Nitric Oxide Does in Your Body

Nitric oxide is a natural signaling molecule in your body that plays a key role in maintaining healthy blood vessels. It helps relax blood vessel walls, improving circulation and keeping blood pressure stable. This test may be used to evaluate how well your body produces nitric oxide, especially in conditions affecting blood flow or inflammation.

When This Test Might Be Ordered

Your healthcare provider might recommend this test if you have symptoms like poor circulation, high blood pressure, or conditions such as peripheral artery disease, Raynaud’s phenomenon, or vascular-related disorders. It may also be part of a broader evaluation for chronic inflammation or certain cardiovascular risks.

How the Test Works

A small blood sample is drawn from your arm and sent to a lab. The sample is analyzed to detect nitric oxide levels, typically measured as nitrite/nitrate, which are stable forms of the molecule. The test does not require fasting and is usually completed within a few days.

What to Expect Before the Test

No special preparation is needed for this test. You may have a slight bruise or soreness at the injection site, but this is normal. If you are on blood-thinning medications, let your provider know before the test.

Reference range

Normal: <100 pg/mLN/A

Specimen: serum

No special preparation required. Inform provider of any chest pain or symptoms.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Nitric Oxide Level

Frequently asked questions

Why might my nitric oxide levels be checked?
Your healthcare provider may check nitric oxide levels to assess vascular health, especially if you have symptoms like cold hands/feet, fatigue, or unexplained high blood pressure. Low levels may indicate reduced nitric oxide production, which could be linked to conditions like atherosclerosis or endothelial dysfunction.
What do my results mean?
Results are not typically provided as a single reference range since nitric oxide levels can vary based on health status, activity level, and other factors. Your provider will interpret the results in the context of your overall health and symptoms. Very low or very high levels may warrant further investigation.
Can lifestyle changes affect nitric oxide levels?
Yes, lifestyle factors such as diet (e.g., Mediterranean diet rich in nitrates), exercise, and avoiding smoking can influence nitric oxide production. Maintaining a healthy weight, reducing stress, and staying active may support optimal levels. Always discuss lifestyle changes with your provider before making significant adjustments.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy