3-Hydroxylysine/Creatinine Ratio, 24-Hour Urine
A 24-hour urine test measuring 3-hydroxylysine relative to creatinine, a marker associated with collagen and connective-tissue breakdown.
["This test can help evaluate collagen and connective-tissue metabolism when a clinician suspects an abnormality."]
Key facts at a glance
- LOINC
- 25933-3
- Short code
- OH-Lysine/Creat 24h Ur-sRto
- Synonyms
- OH-Lysine/Creat 24h Ur-sRto
- Category
- kidney
- Sample
- urine
- Container
- Sterile urine container
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Specimen volume
- 10 mL
- Stability
- Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 4–24 hours
- Order
- routine
What is this test?
This test measures 3-hydroxylysine, a product released during collagen breakdown, in urine collected for 24 hours. The result is compared with the urine creatinine level to help interpret the amount of 3-hydroxylysine.
Why might I need this test?
A clinician may order this specialized test to investigate collagen or connective-tissue metabolism. It may be considered in the evaluation of certain inherited connective-tissue conditions or other situations involving abnormal bone or tissue remodeling.
How is the test performed?
Urine is collected into a designated container for a full 24-hour period, and the 3-hydroxylysine and creatinine measurements are determined from the collection. The laboratory uses the measurements to calculate the reported ratio.
How do I prepare for the test?
Begin the collection at the time specified by the laboratory or clinician and collect every urination for exactly 24 hours. Follow the container, storage, and return instructions provided, and ask whether any diet, supplement, medication, or activity changes are required.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
An elevated 3-hydroxylysine-to-creatinine ratio may indicate increased collagen breakdown or connective-tissue turnover, although injury, supplementation, and other factors can also affect the result. A lower result may reflect reduced tissue breakdown or differences in nutrition, medication use, or metabolism. This test should be interpreted with symptoms, medical history, and other laboratory findings because an abnormal result alone does not establish a diagnosis.
Short name
24-Hour Urine 3-Hydroxylysine/Creatinine Ratio
Frequently asked questions
What does 3-hydroxylysine measure?
Why is creatinine included in the result?
How is a 24-hour urine collection completed?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy