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Hydroxyproline/Creatinine [Mass Ratio], Urine — Turbidimetric Method

Published: August 9, 2026Updated: August 16, 2026

A urine test that measures hydroxyproline relative to creatinine to help assess collagen breakdown and bone turnover.

Quick summary

["This ratio provides an indirect measure of collagen breakdown and bone resorption."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
111224-2
Short code
OH-Proline/Creat Msmt Ur
Synonyms
OH-Proline/Creat Msmt Ur
Category
kidney
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What is this test?

This test measures hydroxyproline in urine and compares it with the amount of creatinine in the same sample. Hydroxyproline is released when collagen is broken down, while creatinine is used to help account for differences in urine concentration.

Why might I need this test?

This test may be used to evaluate collagen breakdown and bone turnover. It has historically been considered in evaluating disorders that affect bone or connective tissue, although it is not a routine or stand-alone bone health test.

How is the test performed?

A urine sample is tested for hydroxyproline using a chemical, turbidimetric method, and the result is compared with urine creatinine. It is usually performed on a spot urine sample rather than requiring an overnight collection.

How do I prepare for the test?

Fasting is generally not required. Follow the laboratory's collection instructions, which may include collecting a clean-catch, midstream urine sample at a specified time.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Urine hydroxyproline/creatinine ratio

Frequently asked questions

Why is hydroxyproline compared with creatinine?
Creatinine helps show how concentrated the urine is. Comparing the two measurements makes the result less dependent on urine concentration.
Do I need to collect urine for 24 hours?
This ratio is usually calculated from a single urine sample. If the laboratory requests a 24-hour collection or an absolute hydroxyproline measurement, follow its specific instructions.
Does this test directly measure bone density?
No. It is an indirect marker of collagen breakdown and bone turnover and cannot replace bone-density imaging or other clinical evaluation.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy