Skip to content

Hydroxyproline/Creatinine Ratio, 24-Hour Urine

Published: August 9, 2026Updated: August 16, 2026

Measures hydroxyproline relative to creatinine in urine collected for 24 hours to help assess collagen breakdown and bone metabolism.

Quick summary

["This urine test provides information about collagen breakdown and may support evaluation of bone and metabolic disorders."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
13763-8
Short code
OH-Proline/Creat Ur
Synonyms
OH-Proline/Creat Ur
Category
kidney
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What is this test?

This test measures hydroxyproline, a substance released when collagen breaks down, in relation to creatinine in a 24-hour urine collection. Creatinine is used as a reference to help account for variations in urine concentration.

Why might I need this test?

A clinician may order this test to evaluate collagen turnover, bone metabolism, or suspected conditions affecting bone or connective tissue. It is sometimes used in the assessment of disorders associated with increased bone breakdown.

How is the test performed?

Urine is collected for a full 24-hour period, and the hydroxyproline and creatinine amounts are measured and used to calculate a ratio. The laboratory analyzes the submitted collection using validated methods.

How do I prepare for the test?

Follow the collection instructions provided by your healthcare professional, including the exact start time, end time, and how to store the container. Tell the laboratory if you take medicines or supplements that may affect collagen or bone metabolism.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-Hour Urine Hydroxyproline/Creatinine Ratio

Frequently asked questions

Why is a 24-hour urine collection needed?
A 24-hour collection captures all urine produced during a complete day and helps reduce the effect of changes in urine concentration. The ratio to creatinine provides additional context for interpreting the hydroxyproline result.
Does this test require fasting?
Fasting is not universally required, but your clinician may give specific instructions based on your situation. Tell the healthcare team about medicines, vitamin supplements, and bone-affecting treatments before collection.
What can affect the result?
Incomplete collections, collection timing, kidney function, hydration, and medicines that influence bone or collagen metabolism can affect interpretation. Your clinician will consider these factors when reviewing the result.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy