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Proline Free Mass Concentration in Urine (24-hour Collection)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of free proline, a non-essential amino acid, excreted in your urine over a 24-hour period. It may help assess kidney function or metabolic conditions.

Quick summary

["This test helps evaluate kidney health and metabolic processes by tracking proline waste in urine."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
111167-3
Short code
OH-Proline Free Msmt Ur
Synonyms
OH-Proline Free Msmt Ur
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms or conditions linked to kidney disease, metabolic disorders, or excessive amino acid buildup. It could also be used in research or specialized clinical studies.

What to Expect Before the Test

You’ll need to collect all urine for a full 24-hour period. Start by emptying your bladder completely at the beginning of the collection period. Store all urine samples in a cool, sealed container until testing. Follow your provider’s specific instructions for timing and handling.

How the Test Works

The collected urine is analyzed to determine the total free proline content. This involves specialized laboratory techniques to measure the concentration accurately, often using advanced methods like chromatography or immunoassay.

What the Results Mean

Results are typically reported as a mass concentration value (e.g., mg/dL or mg/24h). Elevated levels may suggest kidney dysfunction, certain metabolic disorders, or other underlying conditions. Lower than expected levels could indicate errors in collection or specific metabolic issues.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

24-Hour Free Proline Urine Test

Frequently asked questions

What does a high or low result indicate?
High levels of free proline in urine may be associated with kidney disease, where the kidneys fail to properly filter amino acids, or certain metabolic disorders that disrupt amino acid metabolism. However, individual results should always be interpreted by a healthcare professional in the context of your overall health and medical history.
What does it mean if my results are within normal ranges?
If your results fall within the expected range for this test, it generally suggests that your kidney function and amino acid metabolism are functioning as expected based on this specific measurement. However, this does not rule out other potential health issues that might require further testing or evaluation.
How often should I get this test?
The frequency of this test depends entirely on your healthcare provider’s recommendations. They will determine if repeated testing is needed based on your symptoms, medical history, and the results of previous tests. Always follow their guidance for ongoing monitoring.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy