Skip to content

Proline in Urine (Free)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of free proline, an amino acid, in your urine. It may be used to assess kidney function or metabolic conditions.

Quick summary

["Free proline in urine helps evaluate kidney health or metabolic processes."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
32183-6
Short code
OH-Proline Free Ur-sCnc
Synonyms
OH-Proline Free Ur-sCnc
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test if you have symptoms of kidney disease, metabolic disorders, or if you’re being monitored for conditions like prolinemia or other amino acid imbalances. It can also be part of a broader evaluation for unexplained metabolic issues.

What Does the Result Mean?

Results are not typically provided as a single reference range since proline levels vary based on health status, diet, and hydration. Abnormal findings may prompt further testing or evaluation of kidney function or metabolic health.

How Is the Test Performed?

A urine sample is collected, usually over a 24-hour period, to measure free proline levels. The sample is then sent to a lab for analysis using a specialized method, such as high-performance liquid chromatography (HPLC).

What Can Affect Results?

Factors like diet (especially high-protein foods), hydration status, medications, or certain medical conditions (e.g., kidney disease) can influence urine proline levels. Always inform your provider about all medications or supplements you take.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Elevated or abnormal proline in urine may indicate kidney dysfunction, metabolic disorders, or genetic conditions affecting amino acid metabolism. Low levels could suggest dehydration or other metabolic challenges. This test is often part of a broader diagnostic approach rather than a standalone evaluation.

Short name

Urine Proline

Frequently asked questions

What causes abnormal urine proline levels?
Abnormal urine proline levels may result from kidney problems, certain metabolic disorders, or genetic conditions like prolinemia. Dietary factors, hydration status, and medications can also influence results. Always discuss findings with your healthcare provider.
How soon will I get my results?
Results are typically available within 1 to 3 business days, depending on lab processing times. Your provider will explain what the results mean in the context of your health.
Is fasting required for this test?
No fasting is required for this urine test. However, collecting a 24-hour urine sample may require consistency in diet and hydration throughout the period.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy