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Total Protein with Albumin-to-Globulin Ratio (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures your blood’s total protein levels and compares albumin to globulin proteins to help assess protein balance and potential issues like malnutrition or inflammation.

Quick summary

["Your blood’s protein balance and possible health risks can be evaluated with this simple blood test."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
5977-4
Short code
PAI2 Ag PPP IA-mCnc
Synonyms
PAI2 Ag PPP IA-mCnc
Category
chemistry
Sample
plasma
Container
Lavender-top (EDTA) or Green-top (Heparin)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Does This Test Measure?

Total protein includes all proteins in your blood, such as albumin and globulin. Albumin helps maintain fluid balance, while globulins support immune function and clotting. The albumin-to-globulin (AG) ratio compares these two groups to identify imbalances.

Why Might This Test Be Done?

Doctors may order this test to check for conditions like malnutrition, liver disease, kidney problems, or chronic inflammation. It can also help monitor treatment responses for conditions affecting protein levels.

Sample Collection and Preparation

A small blood sample is drawn from your arm. The test uses serum (liquid part of blood after clotting) to analyze protein composition. No special preparation is needed, but fasting may be advised for some related tests.

What Are Common Results and Interpretation?

Results are typically reported as total protein levels and the AG ratio. While exact reference ranges vary by lab, low total protein or abnormal AG ratios may signal protein loss, liver/kidney disease, or other health concerns.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: How Does This Test Help Healthcare Providers?

Short name

Total Protein & AG Ratio

Frequently asked questions

How long does it take to get my results?
Results are usually available within 1–3 business days, depending on your lab. Follow-up with your doctor to discuss findings and next steps.
Can I eat or drink before this test?
No special diet is required, but check with your doctor if they recommend fasting for related tests. Avoid alcohol 24 hours before testing for clearer results.
What if my results are outside the normal range?
If your results show abnormal protein levels or an AG ratio, your doctor may investigate causes like diet, liver/kidney function, or hidden infections. They’ll guide further testing or treatment.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy