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Protein Electrophoresis (Serum/Plasma) with Bromocresol Green Dye Binding

Published: August 9, 2026Updated: August 16, 2026

This test measures the distribution of proteins in your blood, often used to identify abnormal protein patterns, such as those seen in conditions like multiple myeloma or liver disease.

Quick summary

["Helps diagnose and monitor protein-related disorders by analyzing blood protein composition."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
5980-8
Short code
PAI Ag PPP IA-mCnc
Synonyms
PAI Ag PPP IA-mCnc
Category
chemistry
Sample
plasma
Container
Lavender-top (EDTA) or Green-top (Heparin)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

Protein electrophoresis separates blood proteins into different fractions (e.g., albumin, globulins) based on size and charge. Bromocresol green binds to certain proteins, allowing for visual or quantitative assessment of their concentration. This method helps detect imbalances that may signal underlying health issues.

Common Uses and Conditions

This test is typically ordered when doctors suspect conditions like multiple myeloma, liver disease, kidney problems, or certain autoimmune disorders. It may also be used to monitor treatment progress or screen for genetic protein disorders.

Sample Collection and Preparation

A blood sample is drawn from a vein, usually in your arm. The blood is then processed to separate serum or plasma for testing. No special preparation is needed, but fasting may be advised depending on clinical context.

How Results Are Interpreted

Results show the relative amounts of different protein groups. Abnormal patterns (e.g., spikes in certain protein bands) may indicate conditions affecting protein production, storage, or breakdown. Your healthcare provider will review results in relation to your symptoms and medical history.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Protein Electrophoresis (BCG)

Frequently asked questions

Why might my doctor order this test?
Your doctor may recommend this test if you have symptoms like unexplained weight loss, fatigue, frequent infections, or signs of organ dysfunction (e.g., swelling, jaundice). It can also help diagnose hereditary protein disorders or monitor treatment responses in conditions like cancer or liver disease.
What does an abnormal result mean?
An abnormal result may show unusual protein patterns, such as high levels of a specific protein (e.g., monoclonal spikes) or low albumin. These findings can guide further testing or treatment plans, but they don’t diagnose a condition alone. Your doctor will discuss next steps with you.
How often will I need this test?
Frequency depends on your health condition and treatment plan. Some patients may need this test periodically (e.g., every few months) to monitor disease progression or response to treatment, while others may only need it once for screening or diagnostic purposes.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy