Protein Electrophoresis (Serum) with Bromocresol Green and Bromocresol Purple Staining
This test measures the distribution of different protein fractions in your blood serum, helping identify abnormalities in protein levels that may indicate conditions like liver disease, kidney problems, or other metabolic disorders.
["This test helps diagnose and monitor conditions affecting protein balance in your blood."]
Key facts at a glance
- LOINC
- 72521-8
- Short code
- p-ANCA frmln sens IgG SerPl Ql IF
- Synonyms
- p-ANCA frmln sens IgG SerPl Ql IF
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 0
- Ref high
- 17
- Critical range
- 0.5 N/A – —
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- if
- CPT
- 82784
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
What Proteins Does This Test Measure?
Protein electrophoresis separates blood proteins into distinct bands based on size and charge. The test detects key protein groups, including albumin, globulins (such as alpha, beta, and gamma globulins), and other smaller proteins. Each band reflects the concentration of specific proteins, which can vary with health conditions.
Why Might You Need This Test?
Your doctor may order this test to investigate symptoms like swelling (edema), unexplained weight changes, fatigue, or signs of organ dysfunction (e.g., liver or kidney issues). It’s also used to monitor chronic conditions like multiple myeloma, autoimmune diseases, or protein-losing syndromes.
How Is the Test Performed?
A blood sample is drawn from a vein, typically in your arm. The serum portion is separated and placed in an electric field to sort proteins by size and charge. Bromocresol Green and Bromocresol Purple dyes bind to specific proteins, creating visible bands that indicate protein concentrations.
What Does an Abnormal Result Mean?
Abnormal patterns may show too few or too many proteins in certain bands. For example, low albumin could indicate liver disease or malnutrition, while unusual globulin bands might suggest immune disorders or infections. Your doctor will interpret results in the context of your symptoms and medical history.
Reference range
NCBI: NSE 0-17 ng/mLN/A
No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Protein electrophoresis is critical for diagnosing conditions like multiple myeloma (a cancer of plasma cells), chronic liver disease, nephrotic syndrome (kidney disease causing protein loss), and certain autoimmune diseases. It also helps monitor treatment responses and track disease progression by comparing protein patterns over time.
Short name
Serum Protein Electrophoresis
Frequently asked questions
What does a protein electrophoresis test measure?
How long does it take to get results from this test?
Can I eat or drink before this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy