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pH Test

Published: July 31, 2026Updated: August 16, 2026

A pH test measures how acidic or alkaline a sample of your body fluid is by using a special chemical test strip, giving your doctor valuable information about your body's acid-base balance and overall health.

Quick summary

{"pH measures the acidity or alkalinity of body fluid on a scale of 0-14","Normal blood pH ranges from 7.35 to 7.45","Abnormal pH may indicate kidney problems, breathing issues, or metabolic disorders","The test uses a color-changing strip that is compared to a reference chart","Results help doctors diagnose and monitor certain medical conditions"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
50896-0
Short code
pH Fld Strip
Synonyms
pH Fld Strip
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
not specified
Ref low
7.35
Ref high
7.45
Critical range
7.2 not specified – —
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
test-strip
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

The pH test measures the hydrogen ion concentration in your body fluid, indicating how acidic or alkaline (basic) the fluid is. The test uses a chemical test strip that changes color when dipped into the fluid sample. This color is then compared to a standardized chart to determine the pH value.

How is the test performed?

A healthcare provider will collect a sample of your body fluid (such as urine or blood serum). For this test, the sample type is serum, which is the liquid part of blood after it has clotted. The test strip is dipped into the sample, and the color change indicates the pH level. The process is quick and usually takes only a few seconds.

Do I need to prepare for the test?

Your doctor may give you specific instructions. For blood pH tests, you may need to fast (not eat) for a certain period before the sample is taken. For urine pH tests, you may be asked to collect a first-morning sample. Always follow your healthcare provider's instructions and inform them about any medications or supplements you are taking.

What do the results mean?

The pH scale ranges from 0 to 14, with 7 being neutral. Below 7 is acidic, and above 7 is alkaline (basic). For blood (serum), the normal range is tightly controlled between 7.35 and 7.45. Values below 7.35 indicate acidosis, while values above 7.45 indicate alkalosis. Your doctor will interpret your results based on your overall health and other test findings.

Reference range

Varies by fluid type (typically ~7.35-7.45)not specified

Specimen: serum

None required; collect in sterile container without delay

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

FLpH

Frequently asked questions

Is the pH test painful?
The test itself is not painful, but drawing blood may cause brief discomfort or a slight stinging sensation. The test strip itself does not cause any pain.
How long does it take to get results?
Results from a test strip pH test are typically available quickly, often within minutes to a few hours, depending on the laboratory procedures.
What can cause abnormal blood pH?
Many conditions can affect blood pH. Acidosis (low pH) can be caused by kidney failure, diabetic ketoacidosis, lactic acidosis, severe diarrhea, or breathing problems. Alkalosis (high pH) can result from vomiting, diuretic use, hyperventilation, or certain medications. Your doctor will determine the specific cause based on your symptoms and other tests.
Can I test my pH at home?
Home pH test strips are available for urine testing, but they are not a substitute for medical testing. Home tests may not be as accurate as laboratory tests and should not be used for diagnosis or treatment decisions without consulting your healthcare provider.
Do I need to fast before this test?
Your healthcare provider will give you specific instructions. For some pH tests, fasting may be required. Always follow your doctor's pre-test instructions and inform them about any medications you are taking.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy