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Platelet Aggregation with Thrombin-Induced ATP Release (Whole Blood)

Published: August 9, 2026Updated: August 16, 2026

This test measures how platelets release ATP when exposed to thrombin, a protein that helps blood clot. It helps assess platelet function, particularly their ability to respond to clotting signals.

Quick summary

["Evaluates platelet activation and clotting response under controlled conditions."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
56018-5
Short code
Platelet aggr int Bld Thrombin induced ATP secretion.5 U/mL-Imp
Synonyms
Platelet aggr int Bld Thrombin induced ATP secretion.5 U/mL-Imp
Category
hematology
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Ref low
150
Ref high
400
Critical range
50 N/A – 1000 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
thrombin-induced-atp-secretion-u-ml
CPT
85049
SNOMED
900000000000003003
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What This Test Measures

The test evaluates platelet aggregation by measuring the release of ATP, a chemical released when platelets become active. Thrombin is used to stimulate platelet activity, simulating natural clotting signals. The amount of ATP released helps determine how effectively platelets work to stop bleeding.

Why It’s Done

Doctors may order this test if you have symptoms like easy bruising, prolonged bleeding, or unexplained clots. It can also be used before surgeries or procedures that increase bleeding risk, or to monitor conditions affecting platelet function.

Preparation for the Test

No special preparation is needed. You may eat and drink normally before the test. Bring any medications you’re currently taking, as they may affect results.

What to Expect During the Test

A small sample of your whole blood is drawn from a vein, usually in your arm. The blood is then placed in a specialized machine that measures ATP release when exposed to thrombin. The process takes about 15–30 minutes and is generally painless.

Reference range

Adult: 150-400 x 10^9/LN/A

Specimen: whole-blood

No special preparation required. Stay well hydrated.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Platelet Function Test (Thrombin-Induced ATP Release)

Frequently asked questions

What does a high or low result mean?
High ATP release may indicate normal or overly active platelet function, which could be linked to conditions like von Willebrand disease or certain medications. Low ATP release might suggest platelet dysfunction, such as in thrombocytopenia or certain blood disorders.
How does this test compare to other platelet function tests?
This test focuses specifically on ATP release in response to thrombin, which is different from other tests like the Multiplate assay (which measures platelet aggregation in response to multiple stimuli) or light transmission aggregometry (which measures physical platelet clumping). It provides a targeted measure of thrombin-induced platelet activation.
Can this test be affected by medications?
Yes, certain medications like aspirin, clopidogrel, or NSAIDs can interfere with platelet function and may alter results. Always inform your healthcare provider about all medications, supplements, or recent treatments before the test.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy