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Vitamin B6 (Pyridoxine) Plasma Concentration (24-Hour Urinary Excretion)

Published: August 9, 2026Updated: August 16, 2026

This test measures how much vitamin B6 (pyridoxine), a B vitamin essential for nerve function and metabolism, appears in your urine over 24 hours. It helps assess vitamin B6 levels in your body.

Quick summary

["This test helps check if you have enough vitamin B6 or if it’s being lost in urine, which may indicate deficiencies or metabolic issues."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
79325-7
Short code
PLP + PL + Vit B6 Pnl SerPl
Synonyms
PLP + PL + Vit B6 Pnl SerPl
Category
electrolyte
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if you have symptoms like nerve pain, numbness, or unusual fatigue, which could suggest a vitamin B6 deficiency. It may also be used to monitor conditions like epilepsy, anemia, or certain genetic disorders that affect vitamin B6 metabolism.

What to Expect Before the Test

You’ll collect all urine for 24 hours, starting right after you wake up and ending just before you wake up the next morning. Store the urine in a clean container and keep it refrigerated if possible. Your doctor will provide detailed instructions to ensure accuracy.

What the Test Results Mean

Higher-than-normal results might indicate overuse of supplements or certain medications that increase vitamin B6 excretion. Lower-than-normal results could signal a deficiency, especially if combined with other symptoms. Your doctor will interpret results in the context of your overall health.

Common Conditions Monitored

This test is often used to diagnose or monitor conditions like pyridoxine-dependent epilepsy, homocystinuria, or other metabolic disorders affecting vitamin B6 processing.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Vitamin B6 is crucial for brain and nerve function, red blood cell production, and amino acid metabolism. Deficiencies can lead to neurological issues, anemia, or weakened immune function. Urine tests help identify whether vitamin B6 is being properly absorbed or excreted in your body.

Short name

Vitamin B6 Urine Test (24 hr)

Frequently asked questions

How long does it take to get my test results?
Results are typically available within 1–3 business days, depending on your lab’s processing time. Your doctor will contact you once they’re ready to discuss the findings.
Can I take vitamin B6 supplements before the test?
Yes, but follow your doctor’s instructions carefully. Some tests require you to avoid supplements for a set period to get an accurate reading. Always check with your healthcare provider before making changes.
What if my urine test shows abnormal vitamin B6 levels?
If results are low, your doctor may recommend dietary changes, supplements, or further testing to check for deficiencies or underlying conditions. If results are high, they may investigate medications or other factors contributing to the excess excretion.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy