Serum/plasma concentration of free (unbound) estrogen metabolites (24-hour urine)

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms or risk factors for hormone-sensitive conditions, such as breast cancer, endometriosis, or hormonal imbalances. It can also be used to monitor treatment responses in certain cancers or reproductive health conditions.

What to Expect Before the Test

You’ll need to collect all urine for a full 24-hour period. Typically, you’ll start by voiding first thing in the morning and then collect every urine sample in a sealed container until you produce urine again the next morning. Store samples at room temperature until you return them to the lab.

How the Test Works

The collected urine is analyzed to measure specific estrogen metabolites. The test detects free (non-protein-bound) forms of estrogen, which can reflect overall estrogen activity in your body.

Sample Collection Tips

To ensure accurate results, follow these steps precisely: Use a clean container, keep samples refrigerated if not processed immediately, and avoid contamination from soap or other substances. If you miss any urine, contact your lab or provider to adjust your sample collection.