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Pregnenolone and Creatinine, Urine

Published: August 9, 2026Updated: August 16, 2026

Measures pregnenolone in urine along with creatinine, which helps account for variations in urine concentration.

Quick summary

["This test measures urinary pregnenolone and may help evaluate steroid production when interpreted alongside symptoms and other hormone tests."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
30513-6
Short code
Pregntrlone/Creat Ur
Synonyms
Pregntrlone/Creat Ur
Category
kidney
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What is this test?

This test measures the amount of pregnenolone, a steroid hormone and precursor to several other hormones, in your urine. Creatinine is measured at the same time to help account for how concentrated the urine sample is.

Why might I need this test?

A clinician may order this test when evaluating adrenal function, hormone production, fertility concerns, or symptoms that may be related to a steroid-hormone disorder. Urine measurements can help show hormone production over a period of time.

How is the test performed?

A urine sample is collected and analyzed in a laboratory to determine the amount of pregnenolone and creatinine. The clinician may compare the results with a timed collection, such as a 24-hour urine collection, if one was requested.

How do I prepare for the test?

Follow the collection instructions provided by your clinician, including whether a first-morning or timed urine collection is required. Continue prescribed medicines unless your clinician tells you to change them, and mention any supplements or hormone products you use.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Urinary Pregnenolone and Creatinine

Frequently asked questions

What does urinary pregnenolone measure?
It measures the amount of pregnenolone eliminated in urine. Pregnenolone is a hormone-building block used by the body to make cortisol, aldosterone, sex hormones, and other steroids.
Why is creatinine measured with pregnenolone?
Creatinine reflects how concentrated a urine sample is. Including it helps the clinician compare pregnenolone levels with urine concentration and interpret the result more accurately.
Do I need to collect a 24-hour urine sample?
It depends on the order. Some tests use a first-morning sample, while others require all urine collected over 24 hours. Confirm the specific collection method with the laboratory or ordering clinician.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy