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Progesterone and Estradiol, Serum or Plasma

Published: August 9, 2026Updated: August 16, 2026

This test measures the amounts of progesterone and estradiol in blood. These hormones help regulate the menstrual cycle, ovulation, pregnancy, and fertility.

Quick summary

["The results can help evaluate hormonal patterns related to fertility, menstrual irregularity, and ovulation."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
44730-0
Short code
Progest/Estradiol SerPl
Synonyms
Progest/Estradiol SerPl
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82670
SNOMED
300919004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What the test measures

Progesterone supports the uterine lining and is especially important after ovulation. Estradiol is a form of estrogen that supports reproductive function and has effects throughout the body.

Why the test is performed

A clinician may order this test when evaluating infertility, irregular periods, suspected ovulation problems, or symptoms related to a hormonal imbalance. It may also be used to assess hormone patterns during the menstrual cycle.

How the test is performed

A blood sample is collected by venipuncture. The sample is analyzed in the laboratory, and the progesterone and estradiol results are reported with the laboratory's reference information.

Factors that may affect results

Results can vary with the menstrual-cycle day, pregnancy, menopause, hormonal medications, fertility treatments, and certain other medicines. The time of the blood draw and relevant clinical context are important when interpreting the values.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Reproductive hormone panel

Frequently asked questions

Does this test confirm ovulation?
Not always. A progesterone level can support the presence of recent ovulation when interpreted with the cycle timing, but a single measurement may not provide a complete assessment.
Can I take this test while using hormonal medication?
Yes, but hormonal medications, including birth control and fertility treatments, can affect the results. Tell your healthcare professional about all medicines and supplements you use.
Why can the same test have different interpretations at different times?
Progesterone and estradiol normally change during the menstrual cycle. Results therefore need to be evaluated according to the cycle day and clinical situation rather than compared in isolation.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy