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Prolactin Level (24-Hour Profile with HIV Serology)

Published: August 9, 2026Updated: August 16, 2026

This test measures your prolactin hormone level over 24 hours and checks for HIV antibodies in your blood. Prolactin helps regulate breast milk production and affects mood, sleep, and fertility. Elevated levels may signal conditions like pituitary tumors or thyroid issues, while HIV antibodies help detect infection.

Quick summary

["A 24-hour prolactin test and HIV screening may be needed if you have unexplained symptoms or risk factors for prolactin disorders or HIV."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
47870-1
Short code
Prolactin 2.5h p TRH IV SerPl-mCnc
Synonyms
Prolactin 2.5h p TRH IV SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0.8
Ref high
1.2
Critical range
— – 5 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
85610
SNOMED
396252006
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if you have symptoms like unexplained breast milk production in men, infertility, mood swings, or headaches. It could also be recommended if you have a history of pituitary tumors, thyroid disorders, or HIV exposure. A 24-hour profile helps track how prolactin changes throughout the day, while HIV screening ensures early detection of infection.

What to Expect Before the Test

No special preparation is needed for the prolactin test, but you may be asked to avoid certain medications that could affect results. For the 24-hour profile, collect blood samples at regular intervals over 24 hours. For HIV testing, a simple blood draw is typically done. Always follow your healthcare provider’s instructions carefully.

What the Results Mean

Prolactin results alone don’t provide a diagnosis—your doctor will interpret them alongside your symptoms and medical history. Elevated prolactin may suggest conditions like a pituitary tumor or thyroid problems. HIV antibody results are qualitative (positive/negative) and help determine infection status. Discuss any abnormal results with your doctor.

Common Conditions Linked to Prolactin

High prolactin levels can be linked to pituitary adenomas, thyroid disorders (like hypothyroidism), kidney disease, or certain medications. Low prolactin may occur with pregnancy, breastfeeding, or certain medical conditions. HIV infection itself doesn’t directly affect prolactin levels but requires separate monitoring.

Reference range

INR: 0.8-1.2 (warfarin: 2.0-3.0)N/A

Specimen: serum

Inform your healthcare provider if you are taking blood thinners. No special preparation required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Prolactin Profile (HIV Screen)

Frequently asked questions

How often will I need this test?
This test may be done once or repeated if symptoms persist or if further evaluation is needed. Follow-up testing depends on your doctor’s assessment of your condition and results.
What if my prolactin level is high?
A high prolactin level may require further testing, such as imaging (like an MRI) to check for tumors. Your doctor will work with you to determine the best treatment plan, which could include medication or lifestyle changes.
Can I drink alcohol before the test?
Alcohol may not affect prolactin testing, but it can interfere with HIV antibody results. Avoid alcohol for 24 hours before HIV testing if instructed by your provider.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy