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Prolactin level measured 2 hours post-recombinant human growth hormone (rhGH) stimulation via serum/plasma

Published: August 9, 2026Updated: August 16, 2026

This test checks how your body responds to a growth hormone trigger by measuring prolactin levels in your blood after 2 hours.

Quick summary

["Helps diagnose conditions affecting hormone balance or pituitary function."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
44930-6
Short code
Prolactin 2h p TRH IV SerPl-aCnc
Synonyms
Prolactin 2h p TRH IV SerPl-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0.8
Ref high
1.2
Critical range
— – 5 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
85610
SNOMED
396252006
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if they suspect a pituitary gland issue, such as a tumor or disorder that affects hormone production. It can also help evaluate conditions like hyperprolactinemia or growth hormone deficiency.

What to Expect Before the Test

You’ll receive a growth hormone injection (rhGH) at the clinic. Blood will be drawn just before and again 2 hours later to check prolactin levels. Avoid caffeine or stress before testing as it may affect results.

What Happens During the Test

A healthcare provider will draw blood from a vein in your arm. You may feel slight discomfort, but it’s usually over quickly. The blood sample will be sent to a lab for analysis.

What the Results Mean

Results compare your prolactin level before and after the injection. Elevated levels after stimulation may indicate a pituitary issue or other hormonal problems. Your doctor will discuss findings with you.

Reference range

INR: 0.8-1.2 (warfarin: 2.0-3.0)N/A

Specimen: serum

Inform your healthcare provider if you are taking blood thinners. No special preparation required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: Prolactin is a hormone produced by the pituitary gland that regulates milk production and affects other hormones. Elevated prolactin levels can cause infertility, irregular periods, or symptoms like headaches. Low levels may suggest growth hormone deficiency or other pituitary disorders. This test helps diagnose these conditions by assessing how prolactin responds to a growth hormone trigger.

Short name

Prolactin after rhGH test

Frequently asked questions

What does a prolactin level after rhGH stimulation tell me about my health?
It helps identify if your pituitary gland is functioning normally. High levels after stimulation may indicate a pituitary tumor or other issues affecting hormone balance.
How long will I need to wait for my results?
Results typically take 1–2 weeks. Your doctor will contact you with findings once they’re ready.
Can I take anything special before this test?
Avoid caffeine or stress before testing, as they can influence prolactin levels. Follow your doctor’s instructions for fasting or other preparations.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy