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Serum Prolactin Level (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of prolactin, a hormone produced by the pituitary gland, in your blood. Prolactin helps regulate breast milk production and affects other bodily functions.

Quick summary

["Prolactin helps control breast milk production and influences reproductive and non-reproductive behaviors."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
12835-5
Short code
Prolactin 45M p chal SerPl-mCnc
Synonyms
Prolactin 45M p chal SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2
Ref high
18
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84146
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend a prolactin test if you have symptoms like unexplained breast milk production in men, menstrual irregularities, infertility, headaches, or galactorrhea (milk leaking from nipples). It may also be checked to evaluate pituitary gland function or to investigate potential hormonal imbalances.

What to Expect Before the Test

No special preparation is usually needed. You may be asked to fast for 8–12 hours before the test if instructed by your provider. Bring any medications you’re currently taking, as some may affect results. Avoid smoking or drinking alcohol for a few hours before the blood draw.

What Happens During the Test

A healthcare professional will insert a small needle into a vein in your arm. You may feel a slight pinch, but the procedure is quick. Blood is collected into a tube and sent to the lab for analysis. The test typically takes a few minutes.

What the Results Mean

Results are compared to a general reference range, but individual ranges can vary by lab. High prolactin levels may suggest issues like a pituitary tumor, thyroid problems, or medications that affect hormone production. Low levels might indicate pituitary dysfunction or other hormonal disorders.

Reference range

Adult males: 2-18 ng/mL; Females: 2-29 ng/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Prolactin

Frequently asked questions

How often should I get this test?
The frequency depends on your symptoms and medical history. Your healthcare provider will determine how often to repeat the test based on your specific situation, such as monitoring treatment for a pituitary condition or evaluating hormonal changes.
What can affect my prolactin test results?
Certain medications (e.g., antipsychotics, antidepressants, or birth control pills) can raise prolactin levels. Stress, exercise, or certain medical conditions (like thyroid disorders) may also influence results. Always inform your provider about all medications or supplements you’re taking.
Do I need to avoid anything before this test?
Yes, follow your provider’s instructions carefully. Avoid smoking, drinking alcohol, or taking certain medications for a few hours before the test. Some labs may also ask you to fast or avoid strenuous activity, so check with them beforehand.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy