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Prolactin level measured in plasma following a triple bolus administration (pre-test)

Published: August 9, 2026Updated: August 16, 2026

This test measures your prolactin hormone levels in plasma before receiving a series of injections designed to stimulate hormone release. Prolactin is a hormone involved in milk production and may also affect mood, sleep, and reproductive functions.

Quick summary

["This test helps assess how your body responds to hormone-stimulating injections."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
40329-5
Short code
Prolactin pre Triple Bolus SerPl-mCnc
Synonyms
Prolactin pre Triple Bolus SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2
Ref high
18
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84146
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test to evaluate how prolactin levels change in response to specific medications or procedures. It could be used in research or clinical trials involving hormone stimulation protocols.

What Does the Test Measure?

The test measures the concentration of prolactin, a protein hormone produced primarily by the pituitary gland. Prolactin plays roles in lactation, immune function, and may influence other bodily processes like metabolism and mood.

What to Expect Before the Test

You will receive a series of injections (boluses) to stimulate prolactin release. Blood will be drawn just before these injections begin to measure baseline levels. This is typically done in a clinical setting with medical supervision.

What Happens During the Test?

After the baseline blood draw, you will receive injections as part of a controlled study or treatment protocol. Blood samples may be taken at intervals to monitor prolactin levels throughout the process.

Reference range

Adult males: 2-18 ng/mL; Females: 2-29 ng/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Elevated prolactin levels can indicate conditions like pituitary tumors, certain medications, or hormonal imbalances. This test helps monitor how prolactin responds to interventions, which may inform treatment decisions for conditions affecting hormone function.

Short name

Prolactin (Pre-Triple Bolus)

Frequently asked questions

What does a prolactin test measure before the injections?
This test measures your baseline prolactin hormone level in your blood plasma before receiving the hormone-stimulating injections. It helps determine your natural prolactin levels prior to the study or treatment protocol.
Why is this test done before the injections?
The baseline measurement helps establish your starting prolactin level, which is critical for evaluating how your body responds to the injections. It ensures accurate comparison of hormone changes during the study or treatment process.
Can this test be done at home?
No, this test is typically performed in a clinical or laboratory setting. Blood samples are drawn under medical supervision, especially when injections are involved, to ensure safety and accuracy.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy