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Prolactin by Immunoassay (Serum/Plasma)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of prolactin, a hormone produced by your pituitary gland, in your blood. Prolactin helps regulate breast milk production and affects other bodily functions.

Quick summary

["Prolactin helps control milk production and may influence mood, sleep, and fertility."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
12831-4
Short code
Prolactin sp10 p chal SerPl-mCnc
Synonyms
Prolactin sp10 p chal SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2
Ref high
18
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84146
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your doctor may recommend a prolactin test if you have symptoms like unexplained breast milk production (galactorrhea), infertility, irregular menstrual periods, headaches, or signs of hormonal imbalances. It can also help diagnose conditions like pituitary tumors or thyroid disorders that may affect hormone levels.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to avoid certain medications or activities that could influence results. For example, some medications or stress can temporarily raise prolactin levels.

How the Test Works

A blood sample is drawn from a vein, typically in your arm. The sample is then sent to a lab where it is analyzed using an immunoassay method to detect and measure prolactin levels accurately.

What the Results Mean

Results are compared to standard reference ranges, but individual ranges can vary. High prolactin levels may suggest conditions like pituitary tumors or certain medications. Low levels might indicate other hormonal or medical issues.

Reference range

Adult males: 2-18 ng/mL; Females: 2-29 ng/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Elevated prolactin levels can cause symptoms like infertility, menstrual irregularities, galactorrhea, or headaches. It may also mask other hormonal imbalances. Low prolactin levels are less common but can affect fertility and other reproductive functions. Always discuss results with your healthcare provider for personalized interpretation.

Short name

Prolactin Level

Frequently asked questions

How long does it take to get prolactin test results?
Results are typically available within 1 to 3 business days, depending on your lab and healthcare provider’s process.
Can stress or certain medications affect prolactin test results?
Yes, stress, some medications (like antipsychotics or antidepressants), and even certain medical conditions can temporarily raise prolactin levels. Your doctor may ask you to avoid these factors before or after the test if needed.
What are the most common reasons doctors order a prolactin test?
Common reasons include investigating infertility, irregular menstrual cycles, unexplained breast milk production, headaches, or symptoms of hormonal imbalances that may be linked to prolactin levels.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy