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Prolactin, Serum

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of prolactin, a hormone produced by the pituitary gland, in your blood. Prolactin helps regulate milk production and affects other body functions, including reproduction.

Quick summary

["Prolactin levels can help diagnose hormonal imbalances or conditions affecting milk production."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
50517-2
Short code
Prolactin sp1 SerPl-mCnc
Synonyms
Prolactin sp1 SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2
Ref high
18
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84146
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order a prolactin test to check for conditions like hyperprolactinemia (too much prolactin), pituitary tumors, or issues with hormone regulation. It may also be used to monitor treatment for conditions like breast cancer or infertility.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the test if instructed. Wear comfortable clothing and plan to stay for about 15 minutes while the blood is drawn.

What Happens During the Test?

A healthcare professional will insert a small needle into a vein in your arm or hand to collect a blood sample. You may feel a slight pinch, but it usually doesn’t cause significant discomfort. The blood will be sent to a lab for analysis.

What Are Possible Results?

Results are interpreted by your provider based on your medical history, symptoms, and other tests. High or low prolactin levels may signal underlying health concerns that need further evaluation.

Reference range

Adult males: 2-18 ng/mL; Females: 2-29 ng/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Elevated prolactin levels can cause symptoms like missed periods, infertility, breast tenderness, or galactorrhea (milk leakage). Low levels may be linked to conditions like hypothyroidism or certain medications. Your provider will discuss findings in the context of your health.

Short name

Prolactin Level

Frequently asked questions

What causes prolactin levels to be too high?
High prolactin levels can result from pituitary tumors, certain medications (like antidepressants or antipsychotics), thyroid issues, or pregnancy. Lifestyle factors like stress or sleep deprivation may also play a role.
How long does it take to get results?
Results are typically available within 1–3 business days, depending on lab processing times. Your provider will contact you once they’re ready for discussion.
Can diet or exercise affect prolactin levels?
While diet and exercise don’t directly alter prolactin levels, they may influence overall health that affects hormone balance. Avoiding caffeine or alcohol before the test may help ensure accurate results.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy