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Prolactin, Serum (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of prolactin, a hormone produced by the pituitary gland, in your blood. Prolactin helps regulate milk production and affects other bodily functions.

Quick summary

["Prolactin levels can help diagnose hormonal imbalances or conditions affecting the pituitary gland."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
50520-6
Short code
Prolactin sp4 SerPl-mCnc
Synonyms
Prolactin sp4 SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2
Ref high
18
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84146
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Might You Need This Test?

Your doctor may order a prolactin test if you have symptoms like unusual breast milk production, irregular menstrual periods, infertility, headaches, or signs of a pituitary tumor. It may also be used to monitor treatment for certain hormonal disorders or conditions like hyperprolactinemia.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to avoid certain medications or activities that could affect results, such as smoking or certain supplements. Your doctor will provide specific instructions if applicable.

How the Test Is Performed

A blood sample is taken from a vein, typically in your arm, using a small needle. You may feel a slight pinch when the needle goes in, but it usually doesn’t cause much discomfort. The sample is then sent to a lab for analysis.

What the Results Mean

Results are compared to a reference range, which can vary slightly between labs. High prolactin levels may indicate conditions like pituitary tumors, thyroid issues, or certain medications. Low levels might suggest pituitary damage or other hormonal imbalances.

Reference range

Adult males: 2-18 ng/mL; Females: 2-29 ng/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Prolactin Level

Frequently asked questions

What causes prolactin levels to be high?
High prolactin levels can be caused by pituitary tumors, certain medications (like antipsychotics or antidepressants), thyroid problems, kidney disease, or pregnancy. Lifestyle factors such as stress, smoking, or caffeine consumption may also play a role.
How long does it take to get the results?
Results are typically available within 24 to 72 hours, depending on your lab’s processing time. Your doctor will contact you once they have the results.
Can I take anything before my prolactin test?
No special preparations are needed, but avoid smoking, excessive caffeine, or certain medications that might affect results. Always follow your doctor’s instructions for the best accuracy.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy