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Serum Prolactin Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of prolactin, a hormone produced by your pituitary gland, in your blood serum. Prolactin helps regulate breast milk production and affects reproductive functions.

Quick summary

["Prolactin levels can help diagnose hormonal imbalances or conditions affecting the pituitary gland."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
50522-2
Short code
Prolactin sp6 SerPl-mCnc
Synonyms
Prolactin sp6 SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2
Ref high
18
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84146
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms like irregular menstrual periods, infertility, breast tenderness, or unexplained galactorrhea (milk production outside of breastfeeding). It may also be checked in cases of suspected pituitary tumors, thyroid disorders, or certain medications that affect hormone levels.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to avoid certain medications (like some antidepressants or antipsychotics) that could influence prolactin levels. Avoid smoking or caffeine 24 hours before the test, as they might affect results.

During the Test

A healthcare professional will draw blood from a vein in your arm. You may feel a slight pinch, but it usually doesn’t cause significant discomfort. The blood sample is then sent to a lab for analysis.

What the Results Mean

Results help determine if your prolactin levels are within normal ranges or if they’re too high or too low. High levels may suggest conditions like a pituitary tumor or certain medications, while low levels might indicate thyroid issues or other hormonal imbalances.

Reference range

Adult males: 2-18 ng/mL; Females: 2-29 ng/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Prolactin Level

Frequently asked questions

How long does it take to get results?
Results are typically available within 1 to 3 business days, depending on your lab’s processing time. Follow-up with your healthcare provider to discuss the findings.
Can diet or lifestyle affect prolactin levels?
Certain foods, medications, and lifestyle factors—like stress, smoking, or caffeine—can influence prolactin levels. Avoiding these before testing may help ensure more accurate results.
Is this test painful?
The blood draw itself may cause mild discomfort, but it’s generally quick and well-tolerated. Most people don’t experience significant pain afterward.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy