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Proline/Creatinine Ratio, Urine

Published: August 9, 2026Updated: August 16, 2026

Measures the amount of proline relative to creatinine in urine, providing a way to assess proline excretion while accounting for urine concentration.

Quick summary

["This urine test can help identify increased proline excretion, which may occur with certain inherited metabolic or connective-tissue disorders."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
30067-3
Short code
Proline/Creat Ur-Rto
Synonyms
Proline/Creat Ur-Rto
Category
kidney
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What is this test?

This test measures proline and creatinine in a urine sample and calculates their ratio. Proline is an amino acid, while creatinine is a waste product that is commonly used to help account for how concentrated a urine sample is.

Why might I need this test?

A clinician may order this test when symptoms suggest a disorder affecting collagen, connective tissue, or amino-acid metabolism. It can help evaluate whether the body is excreting an unusual amount of proline.

How is the test performed?

A urine sample is analyzed in the laboratory for its proline and creatinine content. The laboratory then calculates the proline-to-creatinine ratio, which reduces the influence of variations in urine concentration.

How do I prepare for the test?

Follow the collection instructions provided by your clinician or laboratory. If a random urine specimen is requested, it is often collected as a clean-catch midstream sample; if a 24-hour collection is requested, all urine should be collected for the specified period.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Urine proline/creatinine ratio

Frequently asked questions

Why is the result reported as a ratio?
The ratio compares proline with creatinine, helping account for differences in urine concentration. This is generally more informative than measuring urinary proline alone.
Does an abnormal result mean I have a genetic disorder?
No. An abnormal ratio can have several causes and must be interpreted with your medical history and other test results. Your clinician can explain whether follow-up testing is appropriate.
Does diet or medication affect the result?
Diet, supplements, illness, and some medications may influence amino-acid excretion. Tell your clinician about supplements, medications, and recent illness before the test.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy