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Proline in Serum or Plasma (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of the amino acid proline in your blood, either drawn as serum or plasma. Proline is involved in collagen synthesis and protein metabolism.

Quick summary

["Proline levels can help assess protein metabolism or be checked in conditions affecting amino acid balance."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
47734-9
Short code
Proline SFr Amn
Synonyms
Proline SFr Amn
Category
chemistry
Sample
fluid
Container
Sterile tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
2-5 mL
Stability
Room temperature: 2h, Refrigerated: 24h
Hemolysis max
300
TAT
4–24 hours
Order
routine

Why Might This Test Be Ordered?

Your healthcare provider may order this test if you have symptoms or conditions linked to amino acid imbalances, such as metabolic disorders, liver disease, or kidney problems. It may also be part of a broader evaluation for nutritional deficiencies or genetic disorders affecting protein metabolism.

What Does This Test Measure?

Proline is an essential amino acid found in proteins and collagen. Your body uses it to build tissues and maintain healthy metabolism. This test specifically looks at the concentration of proline in your blood, which can vary depending on health status, diet, and underlying conditions.

Sample Collection and Preparation

A blood sample is collected from a vein in your arm. The blood is then processed to separate serum or plasma, which is sent to the lab for analysis. This test does not require fasting, but your provider may give specific instructions based on your health.

What Are Common Reasons for Testing?

Tests for proline levels are often done when there is suspicion of conditions like porphyrias, certain genetic disorders, or metabolic issues that disrupt amino acid processing. It may also be used to monitor treatment responses in patients with known metabolic disorders.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Proline Level

Frequently asked questions

What Does a Low or High Proline Level Mean?
Low proline levels might indicate liver disease, kidney problems, or certain metabolic disorders that interfere with amino acid metabolism. High levels could suggest conditions like porphyria or other genetic disorders affecting proline breakdown.
How Does This Test Fit Into My Health?
This test is typically part of a broader diagnostic workup rather than a standalone test. It helps healthcare providers understand how your body processes amino acids and whether there are underlying issues affecting your health.
What Should I Expect Before or After the Test?
No special preparation is usually needed, but avoid alcohol or certain medications that might affect blood chemistry for a few days before testing. After the blood draw, you can resume normal activities immediately. Results may take several days to a week to receive.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy