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Proline in Serum or Plasma (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of proline, an essential amino acid, in your blood. Proline helps maintain healthy skin, connective tissues, and collagen production.

Quick summary

["Proline levels can offer insights into protein metabolism and potential nutrient deficiencies."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
47736-4
Short code
Proline SFr DBS
Synonyms
Proline SFr DBS
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Might You Need This Test?

Your doctor may order this test if they suspect conditions affecting amino acid metabolism, such as certain genetic disorders, liver disease, or kidney problems. It may also be part of a broader evaluation for malnutrition or metabolic disorders.

What Does the Test Involve?

A blood sample is taken from a vein, usually in your arm. The sample is then sent to a lab for analysis using a high-performance liquid chromatography (HPLC) method to measure proline levels accurately.

What Are Possible Results?

Results are typically reported as a concentration value (e.g., in micromoles per liter). While reference ranges vary by lab, abnormal results may indicate imbalances in amino acid metabolism.

What Does It Mean If Results Are Abnormal?

Low proline levels might suggest liver disease, kidney issues, or certain metabolic disorders. High levels could be linked to conditions affecting amino acid processing or storage. Your doctor will interpret results in the context of your overall health and symptoms.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Proline is vital for collagen synthesis and maintaining skin elasticity. Abnormal levels may signal underlying metabolic or organ-related problems, prompting further investigation into conditions like Wilson’s disease, liver cirrhosis, or aminoacidopathies.

Short name

Proline Level

Frequently asked questions

How often is this test performed?
This test is usually ordered as part of a specific diagnostic evaluation rather than a routine screening test. It may be repeated if your condition changes or if additional monitoring is needed.
What do I need to do before the test?
Avoid consuming large amounts of protein-rich foods for 12–24 hours before the test, as this could affect results. Stay hydrated and inform your doctor about any medications you’re taking.
Can diet or supplements affect proline levels?
Yes, dietary intake of proline (found in meats, dairy, and legumes) or certain supplements may influence results. Your doctor may advise dietary restrictions or timing to ensure accurate testing.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy