Protein 24-Hour Urine Collection (Total Protein)

Why This Test Is Done

Your doctor may order this test if they suspect kidney disease, such as nephrotic syndrome, glomerulonephritis, or other conditions causing abnormal protein loss in urine. It can also help monitor kidney function in people with diabetes, high blood pressure, or other chronic health issues.

What to Expect Before the Test

You’ll need to collect all urine for a full 24-hour period. Start by emptying your bladder completely at the beginning of the collection period (e.g., 8:00 AM). Store all urine in a clean container, adding fresh urine every time you urinate. Keep the container refrigerated to preserve the sample. At the end of 24 hours, measure the total urine volume and submit the entire sample to your lab.

During the Test

No special preparation is needed, but you’ll need to follow the collection instructions precisely. The lab will analyze the urine sample for the total protein content.

What the Results Mean

Higher-than-normal protein levels in urine may signal kidney damage or disease. Lower-than-normal levels might indicate dehydration or other conditions affecting protein excretion. Your doctor will interpret the results in the context of your overall health and medical history.