Serum Protein Electrophoresis with Bromocresol Green and Bromocresol Purple Staining

Why This Test Is Done

Your healthcare provider may order this test to check for unusual patterns in your blood proteins. This can help identify conditions like multiple myeloma (a cancer of plasma cells), liver or kidney disease, or certain types of autoimmune disorders. It may also be used as part of a general health check or to monitor treatment progress.

What to Expect Before the Test

No special preparation is usually needed. You may be asked to fast for a few hours before the test, but this depends on your provider’s instructions. Bring any medications you’re currently taking, as some may affect results.

How the Test Is Performed

A blood sample is drawn from a vein, usually in your arm. The blood is then separated into serum (the liquid part after clotting) and sent to a lab. The serum is placed on a gel and run through an electric field to separate proteins by size and charge. Stains like bromocresol green and purple highlight different protein bands.

What the Results Mean

The lab analyzes the pattern of protein bands. Normal results show a balanced distribution of proteins, such as albumin, globulins, and other fractions. Abnormal patterns may indicate low or high levels of specific proteins, which can signal underlying health issues.