Protein Electrophoresis (Serum/Plasma) with Bromocresol Purple Method

What Proteins Does This Test Measure?

Electrophoresis separates blood proteins based on their size and electrical charge. The bromocresol purple dye helps visualize bands corresponding to different protein types. Key fractions include albumin (small, negatively charged), alpha and beta globulins (larger, more complex proteins), and gamma globulins (antibodies). Abnormal patterns may indicate conditions like multiple myeloma, liver disease, or kidney problems.

Why Might You Need This Test?

Your doctor may order this test if you have symptoms like swelling, unexplained weight loss, fatigue, or if they suspect conditions like kidney disease, liver disease, or certain cancers. It’s also used to monitor treatment progress for protein-related disorders.

Sample Collection and Preparation

A blood sample is drawn from a vein, usually in your arm. The blood is then processed to separate serum (liquid part after clotting) or plasma (liquid part before clotting). The sample is sent to a lab where it undergoes electrophoresis to analyze protein distribution.

Common Conditions Evaluated

Abnormal results may suggest conditions like hypoalbuminemia (low albumin), dysproteinemias (abnormal protein patterns), or immune system disorders. For example, high gamma globulins might indicate multiple myeloma or chronic infections.