Protein Electrophoresis (Serum/Plasma) with Bromocresol Purple Dye Shift

What This Test Measures

Electrophoresis separates proteins in your blood based on size and charge. Bromocresol purple binds to proteins differently depending on their concentration, creating distinct bands on a gel. This helps detect abnormal protein patterns, such as monoclonal spikes (seen in multiple myeloma) or elevated globulins (linked to inflammation or liver disease).

Why It’s Done

Your doctor may order this test if you have symptoms like unexplained weight loss, frequent infections, fatigue, or swelling. It’s also used to monitor conditions like kidney disease, liver problems, or cancers that produce abnormal proteins.

Common Conditions It Helps Diagnose

Protein electrophoresis can reveal conditions like multiple myeloma (a cancer of plasma cells), Waldenström macroglobulinemia (a rare blood cancer), or liver/kidney disorders. It may also show signs of chronic inflammation or immune system dysfunction.

Sample Collection and Preparation

A blood sample is drawn from a vein, usually in your arm. The blood is processed to separate serum or plasma, which is then analyzed. No special preparation is needed, but fasting may be advised depending on your doctor’s instructions.