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Serum Protein Electrophoresis with Bromocresol Green (BCG) Staining

Published: August 9, 2026Updated: August 16, 2026

This test measures the distribution of different protein fractions in your blood serum using a specialized dye (bromocresol green) to help identify abnormalities in protein levels.

Quick summary

["This test helps detect conditions affecting protein balance, such as infections, liver disease, or certain blood disorders."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
103812-4
Short code
PSi Scre Ctx EEG
Synonyms
PSi Scre Ctx EEG
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
eeg
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

Electrophoresis separates blood proteins based on size and charge, revealing patterns that can indicate whether proteins are too high or too low in certain categories (e.g., albumin, globulins). Bromocresol green binding highlights specific protein groups, aiding in detailed analysis.

Common Uses and Conditions

Doctors may order this test to diagnose or monitor conditions like chronic inflammation, autoimmune diseases, liver or kidney disorders, or to evaluate protein abnormalities in conditions such as multiple myeloma or nephrotic syndrome.

How the Test Works

A small blood sample is drawn and placed in a gel medium. An electric current is applied, causing proteins to migrate toward the opposite charge. The BCG dye stains certain proteins, creating visible bands that represent different protein types.

Preparation and Procedure

No special preparation is needed, but fasting may be advised depending on your overall health. The procedure is quick and typically done in a lab setting. Results may take a few days to analyze.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

BCG Serum Protein Electrophoresis

Frequently asked questions

Why might my doctor order this test?
Your doctor may recommend this test if you have symptoms like unexplained weight loss, fatigue, swelling, or if they suspect conditions affecting protein levels in your blood, such as liver disease, infections, or certain cancers.
What could abnormal results mean?
Abnormal patterns may suggest conditions like hypoalbuminemia (low albumin), increased globulins (e.g., in chronic inflammation), or specific protein abnormalities linked to diseases like multiple myeloma or autoimmune disorders. Always discuss results with your healthcare provider.
How often should I get this test?
The frequency depends on your health condition and doctor’s recommendations. Some patients may need this test periodically to monitor protein levels or disease progression, while others may only need it once for diagnostic purposes.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy