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Pyruvate-to-Creatinine Ratio, 2-Hour Urine

Published: August 9, 2026Updated: August 16, 2026

Measures the relative amount of pyruvate to creatinine in urine collected over 2 hours. Creatinine helps account for variations in urine concentration.

Quick summary

["This test measures urine pyruvate relative to creatinine and may help evaluate abnormal carbohydrate metabolism when interpreted with other findings."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
25131-4
Short code
PYD/Creat 2h Ur-sRto
Synonyms
PYD/Creat 2h Ur-sRto
Category
kidney
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What is this test?

This test measures pyruvate, a substance formed during the breakdown of carbohydrates, in a 2-hour urine collection. The result is compared with the amount of creatinine in the same urine sample.

Why might I need this test?

A clinician may order this test when symptoms suggest a problem with cellular energy production or carbohydrate metabolism. It may be used alongside other laboratory and genetic tests when evaluating a suspected metabolic or mitochondrial disorder.

How is the test performed?

You collect all urine produced during a specified 2-hour period. The laboratory measures pyruvate and creatinine and calculates their ratio to help account for differences in how concentrated the urine is.

How do I prepare for the test?

Follow the collection instructions provided by your clinician or laboratory, including the exact start and stop times. Unless you are told otherwise, continue your normal diet and fluid intake and do not fast for this test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

2-Hour Urine Pyruvate/Creatinine Ratio

Frequently asked questions

Why is creatinine included in this urine test?
Creatinine is a waste product that is released at a relatively steady rate. Comparing pyruvate with creatinine helps reduce the effect of variation in urine concentration.
How is a 2-hour urine collection obtained?
Empty your bladder at the start of the collection period, then collect all urine produced during the next 2 hours in the container provided. The final urine may need to be collected and included according to the laboratory's instructions.
Does an abnormal result confirm a metabolic disorder?
No. An abnormal ratio alone does not establish a diagnosis. Your clinician will consider it along with your symptoms, medical history, and other laboratory or genetic findings.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy