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Quantitative Immunoassay for Anti-Nuclear Antibodies (ANA) in Serum

Published: August 9, 2026Updated: August 16, 2026

This test measures the presence and level of antibodies that target components of a cell’s nucleus, often used to help diagnose autoimmune conditions.

Quick summary

["Helps detect autoimmune diseases by checking for abnormal antibodies in your blood."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
107734-6
Short code
Quail egg IgG Qn IA
Synonyms
Quail egg IgG Qn IA
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia
CPT
82784
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Is an ANA Test Used For?

ANA tests are primarily used to screen for autoimmune diseases, such as lupus, rheumatoid arthritis, or other connective tissue disorders. They help doctors identify whether your symptoms may be caused by an immune system that’s attacking your own body’s healthy tissues.

How Is the ANA Test Performed?

A small blood sample is drawn from your arm. The serum (liquid part of blood after clotting) is then tested using an immunoassay method to detect and measure anti-nuclear antibodies. Results are reported as either positive or negative, or sometimes as a dilution titer (e.g., 1:40, 1:160).

What Does a Positive ANA Test Mean?

A positive ANA test indicates the presence of antibodies targeting nuclear components, which can suggest an autoimmune condition. However, a positive result alone does not confirm a diagnosis—additional testing and clinical evaluation are needed to determine the exact cause of your symptoms.

Common Conditions Linked to Positive ANA Results

While ANA is not disease-specific, positive results are commonly associated with autoimmune diseases like systemic lupus erythematosus (SLE), scleroderma, mixed connective tissue disease, or rheumatoid arthritis. Other conditions, such as infections or certain medications, can also cause false positives.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

An ANA test is a key screening tool for autoimmune diseases. It helps doctors rule out conditions like lupus or rheumatoid arthritis, guides treatment decisions, and monitors disease activity. However, false positives can occur, so further testing and patient history are essential for accurate diagnosis.

Short name

ANA (Serum)

Frequently asked questions

Why might my ANA test be ordered?
Your doctor may order an ANA test if you have symptoms like joint pain, fatigue, rash, or unexplained fever, which could suggest an autoimmune condition. It’s also sometimes used as part of a routine checkup for individuals at higher risk, such as those with a family history of autoimmune diseases.
What does a negative ANA test mean?
A negative ANA test means no detectable anti-nuclear antibodies were found in your blood sample. While this can rule out certain autoimmune diseases, it doesn’t guarantee you don’t have one. Some autoimmune conditions may have low or variable ANA levels, so further testing may still be needed.
Can lifestyle or medications affect ANA test results?
Yes, certain medications (like hydralazine, procainamide, or minocycline) can cause a false-positive ANA test. Chronic infections or other health conditions may also influence results. Always discuss your medications and health history with your doctor before or after testing.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy