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Guided Stool Microbiome Analysis (16S rRNA Gene Sequencing)

Published: August 9, 2026Updated: August 16, 2026

This test analyzes bacteria and other microbes in your stool to check for imbalances or infections that may affect digestion, immune health, or overall gut function.

Quick summary

["Understanding your gut’s microbial balance can help identify potential health concerns or guide treatment plans."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
35925-7
Short code
RF Guided Stom Endo
Synonyms
RF Guided Stom Endo
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
rf
CPT
86430
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The test examines the diversity and composition of microorganisms (bacteria, viruses, fungi, and archaea) in your stool using genetic sequencing of the 16S rRNA gene. This helps identify which microbes are present and their relative proportions.

Common Uses and Why It Matters

This test is often used when symptoms like persistent diarrhea, bloating, abdominal pain, or unexplained weight changes suggest an imbalance in gut microbes. It may also be used to evaluate conditions like inflammatory bowel disease, food intolerances, or infections.

Sample Collection and Preparation

You’ll provide a fresh stool sample collected at home or in a lab. The sample is then processed to extract DNA from microbes, which is sequenced to identify microbial species.

What to Expect Before the Test

No special preparation is needed, but avoid using stool softeners or laxatives that could alter the sample. If you’re on antibiotics or probiotics, let your provider know, as these can affect results.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Stool Microbiome Profile

Frequently asked questions

How might this test help my doctor?
Your results can reveal whether your gut microbiome is diverse and balanced or if certain microbes are overgrown or missing. This information may help diagnose conditions like small intestinal bacterial overgrowth (SIBO), infections, or conditions linked to gut dysbiosis (microbial imbalance).
What do abnormal results mean?
Abnormal results may indicate an infection, overgrowth of harmful microbes, or a need for probiotics, prebiotics, or dietary changes. Your doctor will interpret results in the context of your symptoms and medical history.
How long will it take to get results?
Results typically take 2–4 weeks, depending on lab processing times. Follow-up with your healthcare provider to discuss findings and next steps.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy