Serum Protein Electrophoresis with Bromocresol Green (BCG) Dye Binding Assay

What This Test Measures

The test uses bromocresol green dye to bind specifically to certain protein groups in your serum. As the dye binds, it shifts color, allowing the lab to separate and quantify proteins like albumin, globulins, and other fractions. This helps identify imbalances that may signal conditions like liver disease, kidney problems, or immune disorders.

Why It’s Done

Your doctor may order this test if you have symptoms like unexplained weight loss, fatigue, swelling, or if they suspect conditions like multiple myeloma, amyloidosis, or chronic liver/kidney disease. It’s also used to monitor treatment progress for protein-related disorders.

Sample Collection & Preparation

A blood sample is drawn from a vein, typically in your arm. The serum (liquid part after clotting) is separated and processed for the dye-binding assay. No special preparation is needed, but fasting may be advised depending on your specific test order.

Common Conditions Monitored

This test helps detect abnormal protein patterns that may indicate systemic diseases, such as: - Protein abnormalities in multiple myeloma or lymphoma, - Liver or kidney dysfunction affecting protein metabolism, - Inflammatory or autoimmune conditions altering protein synthesis.