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Rretrnfl2oclockthicknessoct

Published: August 9, 2026Updated: August 17, 2026

A non-invasive eye imaging test that measures the thickness of the retinal nerve fiber layer to detect optic nerve damage.

Quick summary

{"Uses light waves to create detailed cross-sectional images of the retina","Helps detect and monitor glaucoma and other optic nerve conditions","Quick, painless procedure that takes about 10-15 minutes","Results are compared to normal reference values by age group","Regular testing helps track changes in eye health over time"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
86306-8
Short code
RNFL-2-OCT
Synonyms
R ret RNFL 2 o'clock thickness OCT
Category
cardiac
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
65
Ref high
95
Critical range
40 N/A – 10000 N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 18 years
Method
Optical Coherence Tomography (OCT)
CPT
92134
SNOMED
371632009
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
1–24 hours
Order
routine

What is this test?

Optical Coherence Tomography (OCT) is an advanced imaging test that uses light waves to take cross-sectional pictures of your retina. The retinal nerve fiber layer (RNFL) is a layer of nerve fibers that carries visual information from the retina to the brain. This test specifically measures the thickness of the RNFL at different points around the optic nerve, like a clock face with 12 positions.

How is the test performed?

During the test, you will sit in front of the OCT machine and rest your chin on a support. The technician will align the machine with your eye. You will see a brief flash of light as the scan is taken. The test is completely painless and does not involve any radiation. Your pupils may be dilated with eye drops for better imaging.

How to prepare for the test?

No special preparation is needed. Bring your glasses or contact lenses if you wear them. If your eyes are dilated, you may be sensitive to light for a few hours, so bringing sunglasses is recommended. Avoid wearing eye makeup on the day of the test.

Reference range

Normal Retinal Nerve Fiber Layer (RNFL) thickness for clock hour 2 (temporal-superior) ranges from 65-95 micrometers in healthy adults. Values below 50 micrometers may indicate glaucomatous or optic nerve damage.N/A

Specimen: serum

No special preparation required. Patient should remove eye makeup and contact lenses before imaging. Pupillary dilation may be performed for better visualization.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Does the test hurt?
No, the OCT test is completely painless. You may feel some discomfort from keeping your eye open briefly, but there is no pain or radiation exposure.
How long does the test take?
The actual scanning takes only a few seconds per eye, but the entire appointment including preparation and positioning typically takes about 10-15 minutes.
When will I get my results?
Your doctor will analyze the images and discuss the results with you during a follow-up appointment, usually within a week of the test.
How often should I have this test?
The frequency depends on your condition. For glaucoma patients, testing is typically done annually, but your doctor may recommend more frequent monitoring if there are signs of progression.
What do the results mean?
Results are compared to a database of healthy eyes. Thinner RNFL in certain areas may indicate optic nerve damage. Your doctor will interpret the results based on your specific condition, medical history, and baseline measurements.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy