Understanding Your Medical Test: A Patient's Guide to the Purpose, Procedure, and Meaning of Your Results
A non-invasive eye imaging test that measures the thickness of the nerve fiber layer in the retina to detect early signs of glaucoma and other optic nerve diseases.
{"Uses optical coherence tomography (OCT) to create detailed cross-sectional images of the retina","Measures the thickness of the retinal nerve fiber layer at different clock hours","Helps detect glaucoma and other optic nerve conditions early","The test takes about 5-10 minutes and is painless","Results are compared to normal databases to identify abnormalities"}
Key facts at a glance
- LOINC
- 86307-6
- Short code
- RNFL-3-OCT
- Synonyms
- R ret RNFL 3 o'clock thickness OCT
- Category
- cardiac
- Sample
- serum
- Container
- Not applicable (imaging procedure)
- Units
- N/A
- Ref low
- 48
- Ref high
- 86
- Critical range
- 40 N/A – 10000 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- ≥ 18 years
- Method
- Optical Coherence Tomography (OCT)
- CPT
- 92134
- SNOMED
- 371632002
- Specimen volume
- Not applicable (imaging procedure)
- Stability
- Not applicable (imaging procedure)
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 1–24 hours
- Order
- routine
What is this test? A medical test is a procedure that your healthcare provider orders to check how your body is working, look for signs of illness, or confirm or rule out a specific diagnosis. This overview explains the test in plain, everyday language so you know what to expect before, during, and after the procedure.
The Retinal Nerve Fiber Layer (RNFL) thickness test uses Optical Coherence Tomography (OCT), a advanced imaging technology that uses light waves to take cross-sectional pictures of your retina. This test specifically measures the thickness of the retinal nerve fiber layer around the optic nerve, which is crucial for detecting damage that may not be visible during a regular eye exam.
Why is it performed? Your doctor may recommend this test to help diagnose a new symptom, monitor a condition you already have, screen for health risks before they cause problems, or guide decisions about your treatment. It provides objective information that a physical examination and your medical history alone cannot supply, which helps your care team make better-informed choices.
This test is primarily used to: 1) Detect early signs of glaucoma before vision loss occurs, 2) Monitor the progression of glaucoma or other optic nerve diseases, 3) Evaluate the health of the optic nerve, and 4) help differentiate between various causes of vision loss.
How is the test done? The exact steps depend on the type of test your provider has ordered, but most tests involve either a small sample of blood, urine, or other body fluid, or a simple scan or measurement performed with specialized equipment. A trained nurse or technician carries out the procedure, it is usually quick and causes little or no discomfort, and you can often return to your normal activities right away.
During the test, you will sit in front of the OCT machine and place your chin on a support. The machine will scan your eye without touching it. You will need to look at a fixed target while the technician captures images. The process is quick, painless, and requires no special preparation.
What do the results mean? Your results show whether your measurements fall within the normal reference range for that test. Results within the expected range can be reassuring, while values outside the range may point to a condition that needs closer attention or additional testing. Always review your results with your healthcare provider, who can explain what they mean in the context of your overall health and discuss any next steps in your care.
Your results will show the thickness of your retinal nerve fiber layer measured in micrometers. Thinner than average measurements may indicate optic nerve damage, while thicker measurements can sometimes be seen in certain conditions. Your eye doctor will compare your results to a database of normal eyes and track changes over time.
Reference range
Normal RNFL thickness at clock hour 3 (temporal quadrant) ranges from 48-86 micrometers; values below 50 micrometers may indicate early glaucomatous changesN/A
Patient should remove contact lenses if worn. Pupillary dilation may be performed for better imaging. No special dietary preparations required. Inform technician of any eye surgeries, glaucoma, or optic nerve disorders.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
The RNFL thickness test is crucial for the early detection and management of glaucoma, one of the leading causes of irreversible blindness worldwide. By detecting thinning of the nerve fiber layer before significant vision loss occurs, doctors can initiate treatment to slow or prevent further damage. This test is also valuable in monitoring other conditions such as optic neuritis, ischemic optic neuropathy, and certain neurological disorders that may affect the optic nerve.
Frequently asked questions
Does the test hurt?
Do I need to do anything to prepare for the test?
How long does the test take?
Will I need to have this test again?
Are there any side effects?
Sources
- 1
- 2
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy