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Laboratory Test: What It Is, Why It Is Done, and How to Get Ready

Published: August 9, 2026Updated: August 16, 2026

A non-invasive eye imaging test that measures the thickness of the retinal nerve fiber layer in the upper nasal area of the right eye.

Quick summary

{"Uses light waves to create detailed cross-sectional images of the retina","Measures retinal nerve fiber layer thickness to detect glaucoma damage","Helps monitor changes in optic nerve health over time","Quick, painless procedure taking about 5-10 minutes","No eye drops or radiation exposure required"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
86280-5
Short code
OCT-RNFL-NS-R
Synonyms
R ret RNFL nasal sup thickness OCT
Category
cardiac
Sample
serum
Container
Not applicable (imaging test)
Units
N/A
Ref low
70
Ref high
100
Critical range
50 N/A – 5 N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 18 years
Method
Optical Coherence Tomography (OCT)
CPT
92133
SNOMED
415161008
Specimen volume
Not applicable (imaging test)
Stability
Not applicable (imaging test)
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
24–72 hours
Order
routine

This test is a laboratory analysis, usually performed on a small sample of your blood, urine, or other body fluid. It helps your doctor check how your body is working, screen for certain health conditions, and monitor your overall health over time.

Optical Coherence Tomography (OCT) is an advanced imaging technique that uses light waves to capture detailed cross-sectional images of your retina. This specific test measures the thickness of the retinal nerve fiber layer (RNFL) in the upper nasal area of your right eye. The RNFL contains nerve fibers that carry visual information from your retina to your brain.

Your doctor may order this test to help diagnose a medical condition, find the cause of symptoms you are having, check how well organs such as the liver, kidneys, or thyroid are working, or see how well a treatment is going. It is also often part of a routine checkup even when you feel well.

This test is primarily used to detect and monitor glaucoma, a condition that damages the optic nerve. It can also help identify other optic nerve and retinal disorders. By measuring the RNFL thickness, doctors can detect early signs of damage before vision loss occurs, allowing for earlier treatment and better outcomes.

The test is most often done by drawing a small amount of blood from a vein in your arm. A healthcare professional will clean the skin, wrap a band around your upper arm, and use a thin needle to collect the sample into a tube. You may feel a brief pinch or sting. The sample is then sent to a laboratory, where it is analyzed and the results are reported to your doctor.

During the test, you will sit in front of the OCT machine and place your chin on a support. You will be asked to look at a fixed target while the scanner takes images of your eye. The procedure is completely painless and takes only a few minutes. No eye drops or radiation are involved.

Follow any specific instructions your doctor gives you before the test. You may need to fast, meaning no food or drinks except water, for 8 to 12 hours beforehand. Tell your doctor about all medications, vitamins, and supplements you take, since some can affect the results. Drink plenty of water unless told otherwise, wear a sleeve that rolls up easily, and try to stay relaxed before the blood draw.

No special preparation is needed. You can eat and drink normally before the test. If you wear glasses or contact lenses, bring them with you. Avoid using eye makeup on the day of the test as it may interfere with the imaging.

Reference range

Normal RNFL thickness in nasal superior quadrant: 70-100 microns. Values below 70 microns suggest potential retinal nerve fiber layer thinning. Reference ranges may vary by instrument manufacturer and population.N/A

Specimen: serum

No special preparation required. Remove eye makeup and contact lenses before imaging. Pupillary dilation may be performed for better visualization. Inform technician of any eye conditions, surgeries, or trauma.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings2

Frequently asked questions

Is the OCT test safe?
Yes, OCT is completely safe. It uses light waves, not radiation. There are no known side effects or risks associated with this procedure.
How long does the test take?
The actual imaging takes only 5-10 minutes. Including preparation and time to rest your eyes, plan for about 15-20 minutes at the clinic.
Will I need eye drops?
No, this test does not require eye dilation drops. Your vision may be slightly blurry temporarily from the bright light, but this resolves quickly.
How often should I have this test?
Frequency depends on your condition. For glaucoma patients, testing may be done annually or more frequently as directed by your eye doctor.
Does insurance cover this test?
Most insurance plans cover OCT testing when medically necessary, such as for glaucoma diagnosis or monitoring. Check with your insurance provider for specific coverage details.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy