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Vitamin B12 (Serum) with Quantitative Measurement

Published: August 9, 2026Updated: August 16, 2026

This test measures the level of vitamin B12, a crucial nutrient that helps your body make healthy red blood cells and nerve tissue. Low levels may indicate deficiencies or underlying health conditions.

Quick summary

["Vitamin B12 levels help check for deficiencies that could affect your energy, nerve function, or blood health."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
14527-6
Short code
RUBV Ag Nose Ql IF
Synonyms
RUBV Ag Nose Ql IF
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
if
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Matters

Vitamin B12 is essential for red blood cell production, nerve function, and DNA synthesis. Your body absorbs it primarily from food, and deficiencies can develop over time if intake is insufficient or absorption is impaired. This test helps identify whether your levels are within a healthy range or if further investigation is needed.

What to Expect Before the Test

No special preparation is needed for this test. You may have a blood sample drawn from a vein in your arm by a healthcare professional. If you’re on a vitamin B12 supplement or have recent vitamin B12-rich foods, your provider may advise timing your intake around the test to avoid interference.

Common Causes of Low or High Levels

Low vitamin B12 levels can result from dietary deficiencies, conditions affecting absorption (like pernicious anemia or celiac disease), chronic illnesses, or certain medications. High levels are less common but may occur with long-term vitamin B12 supplements or rare genetic disorders.

Symptoms to Watch For

Symptoms of vitamin B12 deficiency may include fatigue, weakness, numbness or tingling in hands/feet, balance issues, memory problems, or unusual mood changes. If you experience these symptoms, discuss them with your doctor, as early diagnosis and treatment can prevent complications.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Vitamin B12 (Serum)

Frequently asked questions

What does a low vitamin B12 level mean?
A low vitamin B12 level may indicate a deficiency, which can lead to health issues like anemia, nerve damage, or cognitive problems. Your doctor will discuss next steps, such as dietary changes, supplements, or further testing if needed.
Does diet affect vitamin B12 test results?
Yes, your recent diet may influence results. If you’ve consumed vitamin B12-rich foods (like meat, eggs, or fortified foods) or supplements, your doctor might ask you to avoid them for a few days before testing to get an accurate measurement.
Can vitamin B12 levels be high?
Rarely, vitamin B12 levels can be elevated due to long-term supplements or genetic conditions. High levels alone usually aren’t a concern unless accompanied by other symptoms or confirmed by your healthcare provider.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy