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Serum or plasma concentration of vitamin D (25-hydroxyvitamin D)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of vitamin D in your blood, which is important for bone health and other bodily functions.

Quick summary

["Vitamin D levels help ensure you have enough of this essential nutrient for overall health."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
71762-9
Short code
RV IgA Ser Ql IA
Synonyms
RV IgA Ser Ql IA
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia
CPT
82784
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test to check if you have enough vitamin D, especially if you have symptoms like muscle weakness, bone pain, or a history of conditions like osteoporosis or rickets. It can also be used to monitor vitamin D levels if you’re taking supplements or have certain medical conditions that affect vitamin D metabolism.

What Does a Low or High Level Mean?

Low levels of vitamin D may indicate insufficient sun exposure, poor diet, or conditions that interfere with vitamin D absorption. High levels can sometimes be due to overuse of supplements or certain medical treatments.

How Is the Test Performed?

A blood sample is taken from a vein in your arm. The blood is then sent to a lab where it is analyzed to measure vitamin D levels using a specialized immunoassay method.

What Are Common Causes of Abnormal Results?

Abnormal vitamin D levels can result from limited sun exposure, dietary deficiencies, certain digestive disorders, kidney or liver problems, or even medications that affect vitamin D absorption.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Vitamin D is crucial for maintaining bone health, immune function, and muscle strength. Low levels can lead to conditions like osteoporosis or muscle weakness, while very high levels may cause health issues like kidney stones or calcium deposits in soft tissues.

Short name

Vitamin D level

Frequently asked questions

What are the typical reference ranges for this test?
Reference ranges can vary by lab and may include both 25-hydroxyvitamin D (total) and free (unbound) vitamin D. Your results will be compared to the ranges provided by your healthcare provider or lab.
How long does it take to get the results of this test?
Test results are usually available within a few days to a week, depending on your lab’s processing times. Your healthcare provider will contact you once the results are ready.
Can I take vitamin D supplements if my levels are low?
Only follow your healthcare provider’s advice regarding supplements. They will determine the appropriate dose and monitor your levels to avoid excessive intake.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy