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SARS-CoV-2 Nucleocapsid Protein Antigen Qualitative Test (Serum/Plasma)

Published: August 9, 2026Updated: August 16, 2026

This test detects antibodies to the nucleocapsid protein of SARS-CoV-2, the virus responsible for COVID-19. It helps identify if your immune system has recognized and responded to the virus.

Quick summary

This test can help determine past infection with COVID-19 by detecting antibodies in your blood.

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
96899-0
Short code
SARS-CoV-2 ORF1ab Ct OPA wash Qn NAA+prb
Synonyms
SARS-CoV-2 ORF1ab Ct OPA wash Qn NAA+prb
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
CPT
86430
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The test looks for antibodies called nucleocapsid-specific immunoglobulins (IgG and IgM) that your body produces in response to the virus. These antibodies are produced after infection and can remain in your system for months or longer. The presence of these antibodies suggests exposure to the virus, though they may not always confirm a past infection if testing too soon after exposure.

How the Test Works

This is a qualitative immunoassay test performed on serum or plasma. It detects antibodies against the nucleocapsid protein of SARS-CoV-2 using a specific probe. The test result is either positive or negative, indicating the presence or absence of these antibodies.

When to Consider This Test

You may need this test if you have had symptoms of COVID-19, have been exposed to someone with COVID-19, or if you are part of a study or surveillance program. It can also be used in certain clinical settings to assess past infection status.

Limitations and Considerations

While this test can indicate past infection, it may not be accurate if taken too soon after exposure. False negatives can occur if testing is done before your body has had time to produce detectable antibodies. Additionally, some people may not develop detectable antibodies even after infection.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

This test is primarily used for serological screening and confirmation of past SARS-CoV-2 infection. It can be useful in epidemiological studies, for monitoring immunity in vaccinated individuals, and in certain diagnostic algorithms to support clinical decisions. However, it should not replace other diagnostic tests like PCR or antigen tests for current infection.

Short name

Nucleocapsid Antibody Screen

Frequently asked questions

When might I get a false positive or false negative result?
False positives can occur due to cross-reactivity with other viruses or laboratory errors. False negatives are more likely if the test is done too early after exposure, before your body has developed detectable antibodies. Timing of testing and the quality of the test kit can also affect accuracy.
Can this test be used to confirm a recent COVID-19 infection?
No, this test is designed to detect antibodies formed after infection, not the virus itself. For recent infection, PCR or antigen tests are more appropriate. Antibody tests are useful for identifying past infection.
Is this test the same as a COVID-19 vaccine antibody test?
This test looks for antibodies to the nucleocapsid protein, which are produced after natural infection. Vaccine-induced antibodies may target different viral proteins, so they are generally tested separately. Both types of tests can be used together in some clinical scenarios.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy