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SARS-CoV-2 Nucleic Acid Detection by Reverse Transcription Polymerase Chain Reaction (RT-PCR) on Respiratory Specimen

Published: August 9, 2026Updated: August 16, 2026

This test checks for the presence of SARS-CoV-2, the virus responsible for COVID-19, in your respiratory secretions. It helps determine if you have an active infection.

Quick summary

["This test helps identify whether you currently have COVID-19 by detecting the virus’s genetic material in your sample."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
94646-7
Short code
SARS-CoV-2 RdRp Ct Resp Qn NAA+probe
Synonyms
SARS-CoV-2 RdRp Ct Resp Qn NAA+probe
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

How the Test Works

The test uses a small sample of your respiratory secretions (such as nasal or throat swab) to detect genetic material from the SARS-CoV-2 virus. This process involves reverse transcription and polymerase chain reaction (RT-PCR), which amplifies and identifies the virus’s unique genetic sequence.

Sample Collection

A healthcare provider will collect a sample from your nose or throat using a swab. The sample is then sent to a lab for testing. The procedure is quick and generally painless.

When You Might Need This Test

You may need this test if you have symptoms of COVID-19 (such as fever, cough, or shortness of breath), have been exposed to someone with COVID-19, or are required for pre-screening before travel, work, or other activities.

What to Expect After Testing

Results are typically available within 24 to 48 hours, depending on the lab’s processing time. You will receive a call or email with your results from the lab or healthcare provider who ordered the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Positive results indicate an active infection with SARS-CoV-2 and may require isolation, treatment, and monitoring. Negative results suggest no detectable virus at the time of testing, but reinfection or false negatives can occur. This test is critical for guiding public health measures, managing outbreaks, and ensuring safety in communities.

Short name

COVID-19 RT-PCR Test

Frequently asked questions

What does a positive result mean?
A positive result means your test detected genetic material from the SARS-CoV-2 virus, indicating you are likely currently infected and may spread the virus to others.
What does a negative result mean?
A negative result means no detectable virus was found in your sample at the time of testing. However, this does not guarantee you are not infected; you may still have the virus without symptoms or it may take time for the virus to be detected.
Can I get this test at home?
Some at-home COVID-19 tests detect the virus similarly to RT-PCR but are less sensitive and typically used for screening rather than confirmation. For accurate results, healthcare providers or certified labs perform RT-PCR tests.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy